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中文摘要：
为 heparinase 建立一个快速的数量化离子方法我在它在重新组合大肠杆菌关口 i，的生产期间，翻译熔化向量被熔化 heparinase 的 N 终点构造我到绿荧光灯的蛋白质异种(GFPmut1 ) 的 6 号元素碳的化学符号终点。作为结果，不是仅仅是 heparinase 的功能的重新组合表示我在 E。完成的关口 i，而且线性关联在 GFP 荧光紧张之间被获得，允许酶的活性在发酵期间很快被确定并且 heparinase 我活动。
英文摘要：
To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant （GFPmutl）. As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.
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Production of Multienzymes Consisting of Alkaline Amylase and Cellulase by Mixed Alkalophilic Cultur
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