欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
从福寿螺的胃部组织中获得总RNA，经RTPCR扩增、测序得到1条新糖苷水解酶基因片段。分析显示其ORF（Open Reading Frame）全长882bp，编码294个氨基酸。与福寿螺多功能纤维素酶EGX的C端高度同源性达到89．8％，含有糖苷水解酶第10家族的保守性氯基酸序列。
英文摘要：
As a mollusk degrading the plant biomass rapidly, Arnpullaria crossean can produce cellulose-hydrolytic enzymes. This brings us a new sight into the biological degradation of the plant biomass. Total RNA was isolated from the stomach of the mollusk Arnpullaria crossean by using polymerase chain reaction, cloning and sequencing techniques, a complementary DNA DY1300 encoding a low molecular mass glycosyl hydrolase has been identified. It contains a 882 nucleotide ORF encoding a 294 amino-acid protein. The percentage of similarity with the cellulase EGX gene sequence from Arnpullaria crossean is 89.8%. Amino acid sequence-based classification has revealed that the putative protein belongs to the glycosyl hydrolase family 10（GHF10）.
同期刊论文项目
同项目期刊论文
期刊信息
位置：