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中文摘要：
采用RT—PCR（reverse—transcription PCR assay）技术，从里氏木霉QM9414 mRNA中扩增出木聚糖酶基因XYN2，并与质粒pYX212相连，构建了组成型表达载体pYX212／XYN2．将此重组质粒转化入酿酒酵母菌株YPH499进行表达．分析了不同碳源、温度、摇瓶转速、初始pH对其产酶的影响．实验结果表明，最佳碳源是葡萄糖，最佳转速是260r／min，最佳初始pH是6．0．优化重组菌的发酵条件后得到的最大酶活为0．55IU／mL．
英文摘要：
XYN2 gene fragments were successfully amplified from Trichoderma reesei strain QM9414 mRNA by RTPCR （reverse-transcription PCR assay） technology and were ligated to pYX212 vector as the expression vector pYX212/XYN2. Saccharornyces cerevisia YPH499 competent cells were transformed with the recombinant plasmid and began expressing. The effects of different carbon sources, temperature, rotary speed and initial pH on xylanase production of the recombinant were invrstigated. The result indicats that the optimal carbon source is glucose, the optimal temperature is 30 ℃, the optimal rotary speed is 260 r/min, and the optimal initial pH is 6.0. The maximum xylanase activity of the recombinant is 0.55 IU/mL after optimizing the fermentation condition.
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