中文摘要：

目的：检测肿瘤坏死因子相关凋亡诱导配体（TRAIL）在皮肤红斑狼疮（CLE）患者外周血单个核细胞（PBMC）、血清以及皮损组织中的表达，探讨TRAIL与CLE发病的关系。方法：收集28例CLE患者PBMC、血清及皮损组织标本，另取28例健康体检者PBMC和血清、对应正常皮肤组织为对照，运用实时荧光定量聚合酶链反应（Real-Time qPCR）法检测TRAIL mRNA在PBMC和皮损组织的表达，运用酶联吸附免疫法（ELISA）法检测血清sTRAIL浓度、分光光度法检测淋巴细胞半胱氨酸蛋白水解酶（Caspase）-3活性。并对其相关性进行分析。结果：CLE患者与健康对照组PBMC的TRAIL mRNA表达分别为：0．716和0．416，Z=-3．211，P=0．001，血清sTRAIL水平分别为：0．864μg／L和0．621μg／L，Z=-2．004，P=0．045；皮损组织与健康体检者皮肤组织TRAIL mRNA表达分别为：0．448和0．304．Z=-2．164．P=0．030。CLE患者TRAIL mRNA在PBMC表达与在皮损组织的表达、血清sTRAIL水平呈正相关性，P＜0．05。TRAIL mRNA在PBMC和皮损组织的表达与疾病严重程度呈正相关，P〈0．05。CLE患者caspase-3活性为（33．48±7．18）×10^-9nmol／（mg·h），显著高于健康对照组（25．57±4．63）×10^-9nmol／（mg·h），t=4．375，P=0．000，caspase-3活性与PBMC和皮损组织TRAIL mRNA表达呈正相关，P〈0．05，与血清sTRAIL水平相关性无统计学意义，P〉0．05。结论：TRAIL在PBMC、皮损组织、血清中的高表达可能参与了CLE发病，其机制可能与TRAIL通过caspase-3调控细胞凋亡相关。

英文摘要：

Objective： To assess expression levels of TNF-related apoptosis-inducing ligand（TRAIL） in peripheral blood mononu- clear eell（PBMC）, serum and skin lesions of patients with cutaneous lupus erythematosus（CLE）, and to explore pathogenic role of TRAIL in CLE. Methods： Real-time quantitative polymerase chain reaction was used to measure expression levels of TRAIL mR- NA in PBMC and skin lesions of 28 patients with CLE and 28 normal controls. Serum levels of TRAIL were assessed with ELISA, while Caspase-3 activity was measured with spectrophotometry. Results： The expression levels of TRAIL mRNA in both PBMC and skin lesions were significantly higher in CLE patients than in normal controls（0.716 vs 0.416, Z=-3.211, P=0.001 for PBMC; 0.448 vs 0.304, Z=-2.164, P=0.030 for skin）. Moreover, the levels of serum sTRAIL in patients with CLE were also significantly higher than that in normal controls（0.864 μg/L vs 0.621 μg/L, Z=-2.004, P=0.045）. Expression levels of TRAIL-mRNA in PBMC were positively correlated with TRAIL mRNA levels in skin lesions and serum TRAIL levels（P〈0.05）. Expression levels of TRAIL-mR- NA in both PBMC and skin lesions were positively correlated with the severity of the disease（P〈0.05）. Caspase-3 activity was sig- nificantly higher in CLE patients than in healthy controls [33.48±7.18（10-9nmol/mg·h） vs 25.57±4.63（10-9nmol/mg·h）, t=4.375, P= 0.000]. Caspase-3 activity was positively correlated with expression levels of TRAIL mRNA in both skin lesions and PBMC （P〈 0.05）, but not serum TRAIL levels. Conclusion： High expression levels of TRAIL in PBMC, skin lesions and serum may be in- volved in the pathogenesis of CLE, via regulating apoptosis through Caspase-3.