中文摘要：

目的：建立HPLC同时测定了哥王中伞形花内酯、西瑞香素-7-O-β-D-葡萄糖苷、triumbelletin、西瑞香素含量的方法.方法：采用依利特Hypersil-ODS色谱柱（4.6 mm ×200 mm,5μm）,流动相乙腈-0.1％磷酸水溶液梯度洗脱,检测波长345 nm,流速1.0 mL·min-1,柱温30℃,进样量20 μL.比较9个产地了哥王中4种香豆素含量的差异.结果：伞形花内酯、西瑞香素-7-O-β-D-葡萄糖苷、triumbelletin、西瑞香素线性范围分别为0.070～0.770（r=0.9999）,0.190 ～2.09（r =0.9999）,0.765～8.42（r =0.9998）,4.08 ～44.9 mg·L-1（r =0.9999）,平均回收率分别为98.0％ （RSD 1.9％）,96.6％ （RSD 1.2％）,96.5％ （RSD 1.7％）,101.3％ （RSD 1.1％）.不同产地了哥王中triumbelletin和西瑞香素含量明显高于伞型花内酯和西瑞香素-7-O-β-D-葡萄糖苷,伞形花内酯和西瑞香素含量差异较小,西瑞香素-7-O-β-D-葡萄糖苷和triumbelletin含量差异较大,其中云南产地2的西瑞香素-7-O-β-D-葡萄糖苷含量最高,四川产地的含量最低；江西产地的triumbelletin含量最高,云南产地1、广西产地3和贵州产地均未测到triumbelletin.结论：该方法简便、快速、准确,可作为了哥王药材的质量控制方法.

英文摘要：

Objective：To establish a reversed-phase high performance liquid chromatographic method for simultaneous determination of 4 coumarins （umbelliferone,daphnoretin-7-O-β-D-glucoside,triumbelletin and daphnoretin） in Wikstroemia indica.Method：Chromatographic separation was performed on an Elite HypersilODS column （4.6 mm × 200 mm,5 μm） with a gradient elution of acetonitrile-0.1％ phosphoric acid aqueous solution as mobile phase,UV detection wavelength was set at 345 nm,flow rate was 1.0 mL · min-1,column temperature was maintained at 30 ℃ and injection volumn was 20 μL.Difference of four coumarins in W.indica from nine regions was compared.Result：All the standard compounds showed a good linearity in the range of 0.070-0.770 （r=0.9999）,0.190-2.09 （r =0.9999）,0.765-8.42 （r=0.9998）,4.08-44.9 mg·L-1（r =0.9999） for umbelliferone,daphnoretin-7-O-β-D-glucoside,triumbelletin and daphnoretin,respectively.Under these conditions,the average recoveries of these four coumarins were 98.0％,96.6％,96.5％,101.3％,respectively and RSD were 1.9％,1.2％,1.7％,1.1％,respectively.Contents of triumbelletin and daphnoretin were significantly higher than umbelliferone and daphnoretin-7-O-β-D-glucoside in W.indica from nine regions,difference between umbelliferone and daphnoretin was small,but difference between daphnoretin-7-O-β-D-glucoside and triumbelletin was big,the content of daphnoretin-7-O-β-D-glucoside was highest from Yunnan region2,Sichuan region was the lowest; The content of triumbelletin was highest from Jiangxi origin,but this ingredient was not detected from Yunnan region1,Guangxi origin3 and Guizhou origin.Conclusion：This developed method was accurate,sensitive and reproducible,it could be successfully applied to quality control of W.indica.