中文摘要：

为了解鸽特殊的就巢泌乳机理,本研究采用放射免疫技术（RIA）和荧光定量PCR技术对公、母鸽在青年期、就巢期和哺育期3个生理时期,血清中催乳素（PRL）、雌二醇（E2）2种激素浓度的动态变化和催乳素受体（PRLR）、血管活性肠肽（VIP）mRNA在脑、肝脏和肾脏3种组织中的表达变化进行检测。结果表明：在测定期间母鸽血液中2种激素的平均浓度均高于公鸽。在青年期,测定前公鸽血清中PRL和E2水平没有显著变化,但在测定后期（124d）PRL浓度下调,而此时母鸽血清中E2浓度显著提高;就巢期,公、母鸽血清中PRL浓度都有显著升高趋势,并在哺育期达到最高峰,母鸽血清中E2浓度随着就巢时间的延续有下降的趋势,到哺育中期才升高。在鸽脑、肝脏、肾脏3种组织中均表达PRLR和VIP这两种基因,与青年期相比,就巢期公、母鸽脑组织中PRLRmRNA相对表达量显著增加,在哺育第1天达到最高;在就巢期,脑组织中公、母鸽VIPmRNA相对表达量显著高于青年期和哺育期。初步推断PRL对鸽就巢行为的发生和维持起重要作用。

英文摘要：

The aim of this study was to test special broodiness and incubation mechanism of pigeon.The experiment was conducted to investigate changes of serum concentrations of PRL and E2,and the mRNA expression of PRLR and VIP in brain,liver and kidney in male and female pigeon were assayed during the juvenescent phase,broodiness phase and incubation phase by radioimmunoassay（RIA） and real-time PCR.The results showed that,during detection time,the serum concentrations of PRL and E2 in male pigeon were lower than that in female.In adolescence pre-determination,PRL and E2 levels in the male doves did not change significantly.A downward trend of PRL concentration was showed and E2 concentration in female doves significantly increased in later period（124 d）.In reproduction cycle,the concentrations change trend of serum PRL were very similar in male and female pigeons.The serum PRL concentrations in broodiness phase were significantly higher than that in adolescence phase and the expression peak of PRLR occurred in incubation phase.With the passage of time,a down-regulation of the serum E2 concentration was showed,and its concentration increased in mid-incubation phase.The two genes were expressed in three organizations（brain,liver,kidney）.In brain,the expression level of PRLR in male and female pigeons during the broody period were significantly higher than that during the juvenescent phase.In the first day of incubation,the expression of PRLR was expressed maximum.The expression of VIP in brain during the broodiness period were significantly higher than that during the juvenescent and incubation periods.The results suggest that PRL plays an important role in maintaining broodiness behavior of pigeon.