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中文摘要：
评估 Xiongshao 囊的 angiogenic 效果，并且调查在人的脐的静脉 endothelial 房间(HUVEC ) 调停的可能的分子的机制的目的(， XSC ) 它的生物效果。
英文摘要：
Objective： To evaluate the angiogenic effect of the Xiongshao capsule （芎芍胶囊, XSC） in human umbilical vein endothelial cells （HUVEC）, and to investigate the possible molecular mechanisms mediating its biological effect. Methods： Serum pharmacology was applied in this study, in which different doses of XSC were administrated to rats orally and then XSC-containing serum （XSC-S） was collected for the following in vitro experiments. The viability of HUVEC was determined by the 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） assay. Cell density was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with propidium iodide staining was performed to determine cell cycle phase. Cell migration was determined by wound-healing method. Capillary tube formation by HUVEC was examined using ECMatrix gel- based assay. Vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） expression levels were measured by reverse transcription polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbant assay （ELISA） analyses. Results： XSC-S dose-dependently stimulated proliferation of HUVEC by promoting the cell cycle G1 to S progression. In addition, XSC-S treatment dramatically increased the migration and capillary tube formation of HUVEC in a dose-dependent manner. Moreover, XSC-S enhanced the expression of VEGF and bFGF at both mRNA and protein levels. Conclusion： XSC can promote several features of angiogenesis in endothelial cells through up-regulating the expression of bFGF and VEGF, suggesting that XSC may be a potential novel therapeutic agent for the treatment of ischemic heart diseases.
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