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左金丸对人结肠癌多药耐药细胞的逆转作用

ISSN号：1673-1727
期刊名称：中华中医药杂志
时间：2014
页码：844-847
分类：R737.14[医药卫生—肿瘤;医药卫生—临床医学]
作者机构：[1]上海中医药大学附属曙光医院肿瘤科,上海201203, [2]上海市中医医院肿瘤科,上海200071
相关基金：国家自然科学基金（No.81373862,No.81202812）,上海市科委资助项目（No.12140902500）,上海市教委资助项目（No.2011JW57,No.12ZZ118,No.13Y2045）,上海市卫生局科研项目（No.20114Y013,No.2010019）,上海市中医药事业发展三年行动计划（No.ZYSNXD-CC-YJXYY-JS20）
相关项目：健脾解毒方对COX-2激活JNK信号通路介导大肠癌多药耐药的调控研究

关键词：结肠癌, 多药耐药, 凋亡, 左金丸, Colon carcinoma, Multidrug-resistance, Apoptosis, Zuojin Pills

中文摘要：

目的：探讨左金丸对人结肠癌多药耐药细胞HCT-116／L-OHP的逆转作用及其可能机制。方法：人结肠癌HCT-116细胞和HCT-116／L-OHP多药耐药细胞常规培养，左金丸干预48h后，MTT法检测HCT-116／L-OHP细胞对化疗药敏感性的影响，电感耦合等离子质谱法（ICP-MS）检测耐药细胞内奥沙利铂（L-OHP）药物浓度的改变，流式细胞仪分析细胞凋亡情况。结果：左金丸增加HCT-116／L-OHP细胞对奥沙利铂（L-OHP）、顺铂（DDP）、五氟尿嘧啶（5-Fu）、丝裂霉素C（MMC）4种化疗药物的敏感性，4种化疗药的半数抑制浓度（IC50）分别从（157．48±16．73）μg／mL下降至U（71．40±6．48）μg／mL，（26．38±1．92）μg／mL下降到（16．12±1．60）μg／mL，（33．29±3．64）μg／mL下降到（13．11±2．65）μg／mL，（12．50±2．49）μg／mL下降到（2．71±1．10）μg／mL；左金丸干预HCT-116／L-0HP细胞48h后细胞内L-OHP浓度明显增高（P〈0．01），耐药细胞凋亡率显著增加（P〈O．01）。结论：左金丸通过增加结肠癌多药耐药细胞内化疗药物浓度，抑制细胞增殖，诱导其凋亡，逆转HCT-116／L-OHP细胞株的多药耐药性。

英文摘要：

Objective： To investigate the mechanisms and reversal effects of Zuojin Pills on human colon carcinoma multidrug resistance （MDR） in HCT-116/L-OHP cells. Methods： Human colon carcinoma MDR cells HCT-116 and HCT-116/ L-OHP were cultured conventionally. Zuojin Pills were used to intervene them. After 48 hours, the sensitivity to chemotherapy drugs and the reversal effect of multidrug resistance （MDR） in HCT-116/L-OHP cells was evaluated by the MTT assay. The variation of intracellular concentration of L-OHP in HCT-116/L-OHP cells was evaluated by the ICP-MS, and the effects of ZJW on L-OHP induced apoptosis and cell cycle were evaluated by flow cytometry （FCM）. Results： Zuojin Pills could increase the sensitivity of HCT-116/L-OHP cells to L-OHP, DDP, 5-Fu, MMC. The IC50 value of L-OHP decreased from （157.48±16.73）μg/mL to （71.40±6.48）μg/mL, DDP was from （26.38±1.92）μg/mL to （16.12±1.60）μg/mL, 5-Fu was from （33.29±3.64）μg/mL to （13.1 1±2,65）μg/mL, and MMC was from （12.50±2.49）μg/mL to （2.71±1.10）μg/mL. Zuojin Pills increased the concentration of L-OHP in HCT-116/L-OHP cells after treatment for 48h （P〈0.01）. The apoptosis rate of HCT-116/L-OHP cells increased （P〈0.01）. Conclusion： Zuojin Pills reversed MDR in HCT-116/L-OHP cell, which possibly through inhibiting cell proliferation, inducing cell-apoptosis, ultimately affecting the concentration of drugs inside the cells.

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