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小归芍化浊解毒方对胃癌前病变大鼠PCNA及Reg基因干预作用的实验研究

ISSN号：1001-1528
期刊名称：《中成药》
时间：0
分类：R285.5[医药卫生—中药学;医药卫生—中医学]
作者机构：[1]河北省中医院,河北石家庄050011, [2]河北医科大学,河北石家庄050011, [3]广平县人民医院,河北广平057650
相关基金：河北省中医药管理局科研课题（2009014）

作者：王志坤[1], 刘启泉[1], 李博林[2], 董林林[2], 吴云楚[2], 张晓利[1], 苏君[3]

关键词：小归芍化浊解毒方, 胃癌前病变, 增殖细胞核抗原(PCNA), 再生基因I(RegI), Xiaoguishao Decoction , gastric precancerous lesions , proliferating cell nuclear antigen , regeneration gene I

中文摘要：

目的探讨小归芍化浊解毒方（当归、白芍、藤梨根、瓜蒌、茯苓等）对实验性胃癌前病变的作用和机制。方法按随机数字表把100只大鼠随机分成为A（空白）组、B（模型）组、C（中药大剂量）组、D（中药小剂量）组、E（阳性对照）组,每组20只,除空白组外,其余各组采用N-甲基-N-硝基N-亚硝基胍（MNNG）配合饥饱失常诱导造模。造模后A组正常喂养;B组予蒸馏水灌胃;C组按30 g/kg予小归芍化浊解毒方大剂量混悬液灌胃;D组按20 g/kg予小归芍化浊解毒方小剂量混悬液灌胃;E组将胃复春混悬液灌胃。测定胃组织PCNA及RegI的表达量。结果（1）PCNA的表达量：小归芍化浊解毒方大小剂量组与胃复春比较,能明显降低PCNA的表达水平（P〈0.05）。（2）RegI的表达量：小归芍化浊解毒方大剂量组与胃复春组比较,能明显降低RegI的表达水平（P〈0.05）。结论小归芍化浊解毒方可能通过降低PCNA及RegI的表达的影响,逆转胃癌前病变。

英文摘要：

AIM To explore the effect of Xiaoguishao Decoction （Angelicae sinensis Radix, Paeoaiae Radix alba, Aetinidiae argutae Radix, Triehosanthis Fruetus, Poria, etc. ） on gastric carcinoma precancerous lesion. METHODS One hundred normal rats were randomly divided into group A （blank control）, group B （model group）, group C （Xiaoguishao Decoction with large dosage group）, group D （Xiaoguishao Decoction with small dosage group ）, group E （positive control group ） with 20 rats in each group. By using N-methyl-N-nitro-N-nitrosoguanidine in combination with irregular diet, all rats but ones of group A were induced to produce gastric precancerous lesions. After creation of models, group A were feeding normally. The other groups were intragastrieally administrated with distilled water, suspension of Xiaoguishao Decoction with large dosage 30 g herb＇ s extract, suspension of Xiaoguishao Decoction with small dosage 20 g herb＇ s extract, suspension of Weifuchun Tablets, respectively. Then the levels of proliferating cell nuclear antigen （PCNA） and RegI in gastric tissues were tested. RESULTS （ 1 ） The levels of PCNA of group C and D were significantly reduced, and there was significant difference （P 〈 0. 05 ） compared with those of group E. （2） The levels of RegI of group C were significantly reduced, and there was significant difference （P 〈 0. 05 ） compared with those of group E. CONCLUSION The experiment shows that reversal effect of Xiaoguishao Decoction on gastric precancerous lesions is produced.

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