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中文摘要：
目的：研究环孢素A对戊四氮致痫过程中大鼠海马GFAP的影响,并观察大鼠癫痫发作行为变化。方法：72只大鼠随机分为空白对照组（N组）24只,非药物干预组（P组）24只和药物干预组24只（C组）;P组和C组于每日给予腹腔注射戊四氮30mg/kg;同时C组每隔两日给予尾静脉注射环孢素A10mg/kg。采用免疫组化方法观察胶质原纤维酸性蛋白（GFAP）于4d、7d、14d的增生变化;同时观察各组大鼠癫痫发作的时间、发作级别和发作次数。结果：P组大鼠在4d、7d、14d各时间点均未出现癫痫发作时,已经出现胶质细胞增生,并且随着时间的增加胶质细胞增生的程度逐渐升高,与空白对照组（N）比较有显著性差异（P〈0.01）;与C组比较有显著性差异（P〈0.01）;C组大鼠在4d、7d、14d各时间点也均未出现癫痫发作,随着时间的增加胶质细胞增生的程度也逐渐升高,但胶质细胞增生的程度明显低于P组,与P组对应时间点比较,有显著性差异（P〈0.01）;C组大鼠各时间点胶质细胞增生的程度与N组比较也有显著性差异（P〈0.05）。结论：环孢素A干预后大鼠海马星形胶质细胞增生程度降低,减低了大鼠癫痫发作的级别和次数,延迟癫痫发作时间,因此环孢素A具有预防癫痫发作的作用。
英文摘要：
Objective： To study the effects of cycolsporin A （CsA） on glial fibrillary acidic protein （GFAP） expression in hippocampal astrocytes of chronic kindling rats induced by pentylenetrazol（PTZ） and observe the change of the rat behavior. Methods. Seventy--two healthy Wistar rats were randomized into three equal groups ,Group N ,Group P and Group C. Group P and Group C were injected with 35mg/kg PTZ by intraperitoneal every day;Group C was injected with 10mg/kg CsA by vena caudalis every three days. The GFAP expression was determined by immunohistochemical technique in the hippocampi of the rats of each group at 4, 7 and 14 days. Results：The reactive gliosis in the hippocamus was found before the occurrence of PTZ-induced seizure in the Group P,significantly different from those in the Group N and Group C （P〈 0.01） ;the reactive gliosis in the Group C was less than that of Group P,significantly different from those in the Group N （P 〈 0.05） . Conclusion：In the process of chronic kindling induced by PTZ,the GFAP expression in the hippocampal is gradually increased,finally inducing epileptogenesis ;CsA can reduce gliosis in the hippocamus,and therefore,CsA can prevent epileptogenesis.
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