欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
开发了一种共振拉曼光谱（RR）技术用于二价铜离子的定量评价的新方法.通常二价铜离子（Cu~（2＋））在碱性的条件下能够被蛋白质还原成一价铜离子,而两个BCA探针分子能够与一价铜离子发生螯合反应,形成BCA/Cu~＋复合物,它有很强的RR活性.因此,采用这一RR方法进行蛋白质检测,能够比传统方法的检测浓度范围更宽.并且,基于RR方法可以对Cu~（2＋）的最低检测浓度达到5×10~（-7）mol/L,要比传统的比色和紫外可见吸收光谱法更灵敏.该方法提供了一种新颖且高度敏感的RR技术用于金属离子的定量研究.
英文摘要：
In this paper,we have proposed a rapid and highly sensitive Bicinchoninic Acid （BCA） reagent-based Cu2＋ ion quantitation tool using competitive resonance raman （RR） method.A chelation reaction between BCA and Cu＋ which is reduced by protein in an alkaline environment.Two BCA molecules complex with a Cu＋ create a BCA/Cu＋ complex that has strong RR activity.The proposed RR method exhibit a much wider linear concentration range compared to UV -based methods. The detection limits based on the proposed RR method is as low as 5 x 10-7 molJL , which is lower than the traditional method. This novel and sensitive method exhibited an excellent application in quantitative determination of metal ions.
同期刊论文项目
同项目期刊论文
期刊信息
位置：