中文摘要：

在水温（20±1）℃条件下,探讨不同饥饿时间对克氏原螯虾（Procambarus clarkii）消化酶（胃肠组织胃蛋白酶、肝胰脏脂肪酶和胃肠淀粉酶）和抗氧化酶（血清超氧化物歧化酶SOD、肝胰脏过氧化氢酶CAT）的影响。结果表明：随着饥饿时间的延长,胃肠组织胃蛋白酶和肝胰脏脂肪酶活性呈逐渐降低的趋势。饥饿1、2、4d组胃肠组织胃蛋白酶活性分别为（15.80±0.98）、（14.01±1.32）和（13.82±0.93）U,与对照组差异不显著。肝胰脏脂肪酶活性变化趋势更为明显,饥饿2、4d组肝胰脏脂肪酶活性显著低于对照组和饥饿1d组。饥饿8d组胃肠组织胃蛋白酶和肝胰脏脂肪酶活性分别为（12.16±0.44）和（47.43±3.95）U.g-1,显著低于对照组[（16.16±0.23）和（109.98±8.20）U.g-1]。胃肠淀粉酶活性则随饥饿时间的延长呈先升高后降低的趋势,饥饿4d组胃肠淀粉酶活性最高[达（1.89±0.08）U.g-1]。饥饿对克氏原螯虾抗氧化酶活性有显著影响,血清SOD和肝胰脏CAT活性先升高,饥饿1d后迅速降低,饥饿2、4、8d组血清SOD活性分别比对照组下降7.65%、20.37%和28.66%;饥饿2、4、8d组肝胰脏CAT活性分别较对照组下降36.73%、45.58%和63.95%,差异显著。

英文摘要：

This study was conducted to investigate the effect of starvation on major digestive enzymes （gastrointestinal pepsin, hepatopancreatic lipase and gastrointestinal amylase）, antioxidant enzymes [-serum superoxide dismutase （SOD） and hepatopancreatic catalase （CAT）] of the red swamp crayfish （Procambarus clarkii）. The results showed that the activities of gastrointestinal pepsin and hepatopancreatic lipase decreased gradually as the starvation time extended. On the 1st , 2no and 4th day of starvation, the activities of gastrointestinal pepsin were 15.80, 14.01 and 13.82 U respectively, which were approximated to that of the control group （P〉0.05）. The variation trend of the hepatopancreatic lipase activity was more obvious, 2 days and 4 days starvation groups were significantly different from control and 1 day starvation group （P〈0.05）. The gastrointestinal pepsin and hepatopancreatic lipase activities in 8 days group were 12.16 and 47.43 U .g-1 , which showed a significant difference with control （16.16 and 109.98 U . g-1 ） （P〈0.05）. The gastrointestinal amylase activity increased at the beginning of the starvation, and then decreased as the starvation time passed, with its peak on the 4th day of starvation （reached 1.89 U . mg-1 ）. The starvation also significantly influenced the an- tioxidant enzyme activities of P. clarkia as the activities of serum SOD and hepatopancreatic CAT began to rise and dramatically decreased after the first day of starvation. The serum SOD activities of P. clarkii starved for 2, 4 and 8 days were 7.65 %, 20.37 % and 28.66 % lowed than control group, and the hepatopancreatic CAT activities were dropped by 36.73%, 45.58% and 63.95% on the 2nd, 4th and 8＇h day of starvation when compared to control.