欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
为了在短时间内获得相对含量高的磷酸化肽段,以标准磷酸化蛋白质为模型对强阳离子交换色谱（SCX）分离磷酸化肽段体系的缓冲溶液和梯度设置进行了研究,并用酵母酶切肽段混合物考察了该路线在较复杂的样品中的应用。实验结果表明优化后的体系能够在30min内分离出磷酸肽段,而且非磷酸化肽段的干扰很少,这样便相对提高了磷酸化肽段在质谱仪中的响应强度,重要的是该体系可以对复杂样品进行很好的分离。这说明SCX用于规模化磷酸化肽段富集的策略是可行的。本研究为磷酸化蛋白质组学规模化分析提供了实用技术。
英文摘要：
The routine analysis of phosphoproteomics needs effective and specific enrichment methods.The strong cation exchange chromatography（SCX）separation conditions,including the buffer solution and the separation gradient,were studied to enrich the phosphopeptides in a short time.Firstly,the tryptic peptides of standard protein mixtures（bovine α-casein and β-casein,chicken egg ovalbumin）were used to optimize the SCX strategy.Then,this method was applied to the complicated samples,the tryptic peptides from yeast.The experimental results showed that the phosphopeptides were eluted before 30 min in the optimized SCX systems with less interference from nonphosphopeptides.Phosphopeptides were successfully separated in a short time,and the ion signals corresponding to phosphopeptides were accordingly increased during mass spectrometric analysis.It was indicated that the optimized SCX separation system could be used as a simple enrichment method for the separation of phosphopeptides,especially for phosphopeptides in the complex real samples,in a short time,and to increase the relative abundance of phosphopeptides in the detection fraction.This study provides the pragmatic technique for phosphoproteomics analysis on a large-scale.
同期刊论文项目
同项目期刊论文
A method for rapidly confirming protein N-terminal sequences by matrix-assisted laser desorption/ io
Method for quantitative proteomics research by using metal element chelated tags coupled with mass s
Phosphoproteome analysis of the human Chang liver cells using SCX and a complementary mass spectrome
A method for rapidly confirming protein N-terminal sequences by matrix-assisted laser desorption/ion
Highly efficient enrichment of phosphopeptides by magnetic nanoparticles coated with zirconium phosp
Enrichment of phosphopeptides by Fe3+-immobilized magnetic nanoparticles for phosphoproteome analysi
Specific capture of phosphopeptides on matrix-assisted laser desorption/ionization time-of-flight ma
Systematic characterization of the covalent interactions between epigallocatechin gallate and peptid
The miniaturization of bioanalytical assays and sample pretreatments by exploiting meso-fluidic lab-
Selective isolation of acidic proteins with a thin layer of multi-walled carbon nanotubes functional
One system with two fluorescence resonance energy transfer (FRET) assembles among quantum dots, gold
Fiber-optic fluorescence sensor for dissolved oxygen detection based on fluorinated xerogel immobili
Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with ex
Isolation of hemoglobin from human blood by solid phase extraction with lanthanum(III) modified zeol
A pH-responsive poly(N-isopropylacrylamide -co-acrylic acid) hydrogel for the selective isolation of
Poly(phthalazine ether sulfone ketone) as novel stationary phase for stir bar sorptive extraction of
Biological cell-sorption for separation/preconcentration of ultra-trace cadmium in a sequential inje
New developments in flow injection/ sequential injection on-line separation and preconcentration cou
Selective extraction of low molecular weight proteins by mesoporous silica particles with modified i
Functionalization of multiwalled carbon nanotubes with hyperbranched polyethyleneimine for highly se
A highly fluorescent hydrophilic ionic liquid as potential probe for the sensing of biomacromolecule
A new poly (phthalazine ether sulfone ketone)-coated fiber for solid-phase microextraction to determ
Surface-assembly of graphene oxide nanosheets on SiO2 particles for the selective isolation of hemog
Combination of Improved 18O Incorporation and Multiple Reaction Monitoring: A Universal Strategy for
Coating a thin layer of NaBH4 solution for mercury vapor generation coupled to atomic fluorescence s
期刊信息
位置：