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中文摘要：
在特定实验条件下，蚕丝蛋白对细胞色素c表现出选择性吸附．以蚕丝蛋白微填充柱为载体，在流动系统中建立了细胞色素c的分离富集方法，以分光光度法在410nm处检测分离富集过程．在进样流速低于10μL／s时，2mL样品溶液（pH=5．6的水溶液）中5μg／mL的细胞色素c可被蚕丝蛋白微柱完全吸附，而在洗脱流速低于15μL／s时，200μL NaCl溶液（1．0mol／L）可将吸附的细胞色素c完全洗脱，分离富集系数为10．用本方法测定细胞色素c的线性范围为1．0～10．0μg/mL，检出限为0．33μg/mL，精密度RSD为2．5％（5μg/mL，n=9）．此外，还采用本文方法对人全血中的蛋白质进行了分离富集，并用SDS-聚丙烯酰胺凝胶电泳验证了分离后蛋白的纯度．
英文摘要：
Selective adsorption of cytochrome c using silk fibroin as adsorbent was observed under certain experimental conditions. A novel separation and preconcentration approach for cytochrome c was thus developed via a silk fibroin packed micro-column in a sequential injection system. The entire process of separation and elution was monitored by spectrophotometry at 410 nm. At pH 5.6, cytochrome c in 2 mL of aqueous solution at 5 g/mL were quantitatively adsorbed by the silk fibroin micro-column, and a NaC1 solution of 1.0 mol/L suffices the complete elution of the adsorbed cytochrome c from the micro-column. An enrichment factor of 10 was achieved by fixing a sample volume of 2 mL and an eluent volume of 200 μL. A linear calibration graph within 1.0-10. 0 μg/mL cytochrome c was obtained with a detection limit of 0.33 μg/mL and a precision （RSD） of 2.5 % （5 μg/mL, n = 9 ）. The practical applicability of this system was demonstrated by processing human whole blood for preconcentration of cytochrome c, and the results were verified by assay with SDSPAGE.
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