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中文摘要：
该技术的成功基质辅助激光解析电离飞行时间质谱（MALDI-TOFMS）技术已成为目前蛋白质组学研究中的经典技术。在应用过程中，合适的样品前处理方法起着首要和关键的作用。只有将合适的样品前处理方法与合适的有机基质结合起来，才能成功实现对多肽和蛋白质等生物大分子的准确鉴定。随着质谱分析向高灵敏度、高准确度、高通量的方向发展，出现了许多新的，可直接在MALDI靶上前处理的方法，极大地简化了对生物样本的处理步骤，同时减少了样品的损失。特别是将一些纳米材料用于MALDI靶上进行样品的前处理，为样品前处理方法的研究开辟了广阔的应用前景。
英文摘要：
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） became a classical tool for the proteome analysis. In the analytical process, proper pre-treatment method of samples, the same as the selection of matrix played a major role for the successful identifications of proteins and peptides. As sensitivity, mass accuracy and throughput of MS analysis become important parameters, lots of pretreatment methods on-probe, including those promising protocols based on nano-particles materials, which have been used to improve the analysis of biological samples.
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