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中文摘要：
理解房间的极端结构的反应到结冰过程为为房间和纸巾设计 cryopreservation 策略是重要的。依附的房间的细胞的结构是导致 cryopreservation 的损坏的目标。特定的荧光染色被用来估计纳入 hydroxyapatite 磁盘的鼠科的造骨细胞的肌动朊细丝(F 肌动朊) 的地位，塑料盖子为冻结过程的一个二拍子的圆舞滑倒。死了的房间的 F 肌动朊被使解聚并且在结冰过程弄歪，而实时房间的有小变化。结果建议细胞骨架可以在 cryopreservation 期间支持房间的坚韧性。现在的学习帮助在结冰过程期间调查依附的房间的损坏机制。
英文摘要：
Understanding the ultrastructural response of cells to the freezing process is important for designing cryopreservation strategies for cells and tissues. The cellular structures of attached cells are targets of cryopreservation-induced damage. Specific fluorescence staining was used to assess the status of the actin filaments （F-actin） of murine osteoblasts attached to hydroxyapatite discs and plastic coverslips for a two-step freezing process. The F-actin of dead cells was depolymerized and distorted in the freezing process, whereas that of live cells had little change. The results suggest that the cytoskeleton may support the robustness of cells during cryopreservation. The present study helps to investigate the damage mechanism of attached cells during the freezing process.
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