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中文摘要：
[目的]探讨自然杀伤T细胞（NKT）在不同证型活动期与缓解期溃疡性结肠炎（UC）患者中的差异表达及其临床意义.[方法]将UC患者分为以下4组：湿热型活动期组（A）、湿热型缓解期组（B）、脾肾阳虚型活动期组（C）、脾肾阳虚型缓解期组（D）,各25例;选取20例行肠镜检查的健康体检者作为对照组.采集以上5组的外周血及结肠黏膜组织,采用流式细胞术检测NKT及其细胞因子γ干扰素（IFN-γ）和肿瘤坏死因子α（TNF-α）的频率.[结果]①NKT：外周血及结肠频率均呈现A＜B＜C＜D的顺序,且A显著低于B,C显著低于D,A显著低于C,B显著低于D（均P＜0.05）;每组结肠NKT频率均显著低于外周血（P＜0.05）.②细胞因子：外周血与结肠频率大体呈现A＜B＜C＜D,且组间差异有统计学意义,且每组结肠NKT频率均显著低于外周血（P＜0.05）.[结论]NKT及其细胞因子频率降低在湿热证型及活动期UC发病机制中起到关键作用.
英文摘要：
[Objective]To study the expression and its clinical significance of natural killer T （NKT） cells in different syndrome types of patients with ulcerative colitis （UC）.[Methods] Patients with UC were divided into the following four groups：active syndrome group （A）,syndrome remission group （B）,spleen-kidney Yang deficiency type activity group （C）,spleen-kidney Yang deficiency type remission group （D）,25 cases each.Twenty healthy people who underwent colonoscopy examination were selected as control group.Peripheral blood and colonic mucosa tissue of the 5 groups were obtained.Flow cytometry was used to detect NKT and cytokines gamma interferon （IFN-gamma） and tumor necrosis factor alpha （TNF alpha） frequency.[Results] ①The NKT：peripheral blood and colon frequency of NKT were presented as A ＜B＜C＜D order,and A was significantly lower than B,C lower than D,A lower than C,and B lower than D （all P＜ 0.05）.Colon NKT frequency in each group was significantly lower than peripheral blood （P＜ 0.05）.② Cytokines：peripheral blood and colon frequency of cytokines were presented as A＜B＜C＜D,and there were also statistical differences between groups.Colon of NKT frequency in each group was significantly lower than peripheral blood （P＜ 0.05）.[Conclusion] The NKT and reduced frequency of cytokines play a key role in damp-heat syndrome type and active UC pathogenesis.
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