中文摘要：

以北京地区11个牧场的494头中国荷斯坦牛为试验材料,采用病例-对照（case-control）设计,研究了motilin（MLN）基因第一外显子内转录因子结合位点g.90T〉C多态性与奶牛真胃变位易感性的关联。结果共检测到TT、TC、CC三种基因型,基因型频率分布符合Hardy-Weinberg平衡（P〉0.05）。将该位点3种基因型与真胃变位易感性进行关联分析,结果发现在一胎牛中关联显著（Cochran-Armitage趋势检验P〈0.05;卡方检验P=0.09）;与基因型TT相比,基因型TC和CC的比值比（OR值）分别为1.52和2.06,即易感性CC〉TC〉TT,显示C等位基因更易导致真胃变位。这些结果支持了MLN基因与荷斯坦牛真胃变位可能存在密切的关系。对g.90T〉C位点T等位基因的选择,将有利于提高群体对真胃变位的遗传抗性。

英文摘要：

The association between a transcription factor binding site polymorphism （g.90T〉C） in the exon 1 of bovine MLN gene and abomasal displacement in dairy cattle was investigated using a case-control design. A total of 494 Chinese Holstein cows were collected from 11 herds in Beijing. Three kinds of genotypes （TT, TC, CC） were identified and no significant departure from Hardy-Weinberg equilibrium was detected （P〉0.05）. Significant association between the SNP and abomasal displacement was found for the first lactation cows （Cochran-Armitage trend test P〈0.05; chi-square test P=0.09）. Odds ratios （OR） for TC and CC were 1.52 and 2.06, respectively, with TT as reference, indicating the increasing susceptibility of cows with TT, TC, and CC. These results suggested a significant genetic association between MLN gene and abomasal displacement in Chinese Holstein cattle. Selection for the favorable T allele of g.90T〉C could potentially improve genetic resistance to abomasal displacement.