中文摘要：

目的：探讨红车轴草提取物（Trifoliumpratense Leguminosae extract，TLE）对小鼠T淋巴细胞的体外活化和增殖的影响。方法：MTT比色法检测TLE对细胞的毒副作用及增殖抑制情况；双色荧光抗体染色结合流式细胞术分析TLE对小鼠T淋巴细胞在多克隆刺激剂（ConA）刺激下体外活化抗原CD69表达的影响；羧基荧光素乙酰乙酸（carboxyiluorescein diacetate，succinimidyl ester，CFDA—SE）标记技术结合流式细胞术，分析CD3^＋T淋巴细胞增殖相关指数。结果：TLE对小鼠淋巴结来源的淋巴细胞微毒副作用；终质量浓度为10、20、30、40mg／L的TLE对ConA刺激下的T淋巴细胞体外活化抗原CD69表达具有明显的抑制作用（P〈0．01），40mg／L抑制达到峰值；MTT法和CFDA—SE染色法都显示，10、20、30、40mg/L TLE对ConA诱导的T淋巴细胞增殖具有显著的抑制作用（P〈0．01）。结论：TLE对小鼠T淋巴细胞的体外活化和增殖具有明显的抑制作用。

英文摘要：

Aim：To study the effect of Trifoliumpratense Leguminosae extract（TLE） on activation and proliferation of mouse T lymphocytes in vitro. Methods： The effect of TLE on the side-effect and proliferation of the murine lymphocytes were estimated by MTT test;Double-fluorescent antibody staining plus flow cytometrics were used to detect CD69 expression;CFDA-SE staining CD3^＋ T lymphocytes proliferation. Results： phocytes at the mass concentration of 10.20 plus flow cytometrics to analyze d proliferation of mouse T lymlittle side-effect on mouse lymphocytes. Conclusion： TLE can inhibit the activation and proliferation of mouse T lymphocytes in vitro