中文摘要：

目的：研究清开灵注射液（QKL）体外抑制HIV-1的作用。方法：用MTT比色法检测QKL对H9/HIV-1ⅢB细胞（慢性感染HIV-1ⅢB的H9细胞）和MT-2细胞的毒性;采用荧光染料Calcein-AM标记的H9/HIV-1ⅢB细胞和系列稀释的QKL作用后,与MT-2细胞混合培养,2 h后在荧光下计数融合细胞数目,评价QKL对两种细胞早期融合的影响;用MTT法检测QKL对HIV-1ⅢB急性感染的MT-2细胞的保护作用;用HIVp24抗原试剂盒检测HIV-1ⅢB感染的MT-2细胞的培养上清p24抗原的含量,分析QKL对HIV-1复制的影响。结果：QKL对H9/HIV-1ⅢB细胞和MT-2细胞的CC50分别为1/50.76和1/36.97;抑制H9/HIV-1ⅢB细胞和MT-2细胞早期融合作用的EC50=1/235.29,SI=6.36;对HIV-1ⅢB感染的MT-2细胞保护作用的EC50=1/144.93,SI=3.92;抑制p24抗原产生作用的EC50=1/175.44,SI=4.75。结论：QKL体外有抗HIV-1活性,作用机制可能是多靶点的,可抑制病毒进入细胞和胞内复制。

英文摘要：

Aim： To investigate the inhibitory effect of Qingkailling（QKL） on HIV-1 in vitro. Methods： The cytotoxicity of QKL was detected by MTT assay. H9/HIV-1 ⅢB cells labeled calcein-AM were treated with serially diluted QKL, and then co-cultured with MT-2 cells. After 2 hours, the cell fusion was observed with a fluorescencmicroscope. The protective effect of QKL on MT-2 cells infected by HIV- 1 ⅢB was detected by MTT. Viral replication was analyzed by measuring the level of p24 antigen in culture supernatants by p24 kit. Results： QKL was toxical to H9/HIV-1 ⅢB cells and MT-2 cells. The CC50 was 1/50.76 and 1/36.97 respectively. The EC50 of QKL against early Hg/HIV-1 ⅢB ceils and MT-2 ceils fusion was 1/235.29 with a selective index of 6.36. QKL could protect MT-2 ceils direct infected by HIV- 1 ⅢB from death. The ECso was 1/144.93 with SI 3.92. The EC50 of QKL against P24 antigen production was 1/175.44 with SI of 4.75. Conclusion： QKL has inhibitory effect on HIV-1 in vitro and it reacted through at least two clarified antiviral mechamisms. QKL inhibited not only entry of HIV-1 into cell but also the replication.