中文摘要：

目的：研究黄芩苷（BA）抑制Jurkat T细胞（人T淋巴细胞白血病细胞株）的生长和诱导其凋亡的作用及机制。方法：以噻唑兰（MTT）比色法测定BA对细胞生长的抑制率；胞内[Ca^2＋]i和线粒体跨膜电势（△ψm）分别用Fluo-4／AM和DiOC6（3）荧光探针标记，流式细胞仪检测；PI染色流式细胞术分析细胞周期分布及凋亡率；凋亡细胞形态以Hoechst33342／PI染色后荧光显微镜观察。结果：BA明显抑制Jurkat细胞增殖（P〈0．01），并呈时间、浓度依赖性；BA可浓度依赖的诱导Jurkat细胞[Ca^2＋]i浓度上升、线粒体膜电势△ψm降低，将细胞周期阻滞在S期，凋亡率增加，荧光显微镜下可见经BA（100mg／L）作用的细胞呈现典型的凋亡核固缩表现，胞核呈致密浓染的颗粒状荧光。结论：BA体外显著抑制Jurkat T细胞增殖及诱导其凋亡，其机制可能与胞内[Ca^2＋]i及线粒体依赖的凋亡通路有关。

英文摘要：

Aim：To investigate the effects and mechanism of baicalin （BA） on growth inhibition and inducing apoptosis of Jurkat T cell（ human T lymphocyte leukemia cell line） in vitro. Methods： The inhibitory effect of BA on growth of Jurkat cells was measured by MTT assay. Intracellular free Ca^2＋ （ [ Ca^2＋ ]i） and mitochondrial membrane potential （△ψm ） in ceils were labeled with Fluo-4/AM and 3, 3＇-dihexyloxycarbocyanine iodide [ DiOC6 （3） ] and tested by flow cytometry （FCM）. The distribution of the cell-cycle and apoptosis rates were analyzed by propidium iodide （PI） staining together with flow cytometry. Observe the morphological changes of the apoptosis ceils by fluorescence microscope with Hoechst33342 and PI staining. Results： BA could inhibit the growth of Jurkat ceils significantly in a time- and dose-dependent manner（P 〈 0. 01 ）. BA induced apoptosis, [ Ca^2＋ ] i increase and the decrease of △ψm of Jurkat ceils in a dose-dependent manner, and led the cell-cycle to S arrest（ P 〈 0. 05 ）. The morphological changes of apoptosis, such as nuclear fragmentation or condensed particles were identified after incubation by BA（ 100 mg/L）. Conclusion： BA showed the effects on growth inhibition and inducing apoptosis of Jurkat ceils, the mechanism of the action resulted in activating mitochondria and Ca^2＋ signaling pathway.