中文摘要：

目的：研究中药喘可治注射液（CKZ）对小鼠腹腔巨噬细胞凋亡、一氧化氮（NO）释放和细胞因子分泌的影响,并探讨其作用机制。方法：无菌分离小鼠腹腔巨噬细胞,制备单细胞悬液,加入CKZ（终体积分数20μL/mL）孵育4 h后,加入过氧化氢（H2O2）、放线菌酮（CHX）、环磷酰胺（CTX）诱导细胞凋亡,荧光酶标仪结合SytoxGreen染色检测细胞凋亡;加药孵育4 h后,加入细菌脂多糖（LPS,终质量浓度10μg/mL）和IFN-γ（终质量浓度80ng/mL）,24h后Grass试剂盒检测巨噬细胞NO的量;加药孵育4 h后,加入LPS（终质量浓度10μg/mL）和IFN-γ（终质量浓度80 ng/mL）,24h后流式细胞仪结合Cytometric Bead Array（CBA）技术检测细胞因子的释放。结果：CKZ能抑制H2O2、CHX、CTX诱导的细胞凋亡;促进非刺激状态下巨噬细胞NO和IL-6、IL-10、MCP-1、IFN-γ、TNF-α5种细胞因子的产生,抑制LPS和IFN-γ刺激的NO和IL-6、IL-10、MCP-1、IFN-γ、TNF-α5种细胞因子的产生。结论：CKZ可抑制巨噬细胞凋亡,并对巨噬细胞NO的分泌和IL-6、IL-10、MCP-1、IFN-γ、TNF-α5种细胞因子的生成具有双向调节作用。

英文摘要：

Aim：To investigate the effect of Chuankezhi（CKZ） on apoptosis,NO release and cytokine secretion of murine peritoneal macrophages in vitro,and the mechanism of its action. Methods： The single cell suspension of murine peritoneal macrophages was prepared under sterile condition.After preincubated with CKZ for 4 hours,H2O2、CHX、CTX were added to induce cell apoptosis.24 hours later,sytox green plus fluorescence microplate rader was used to detect apoptosis of the cells.After pre-incubated with CKZ for 4 hours,the cells were stimulated with LPS at a final concentration of 10 10μg/mL and IFN-γ at a final concentration of 80ng/mL.24 hours later,the amount of NO produced in macrophages was detected by Griess kit,and release of cytokines was detected by cytometric bead array（CBA） plus flow cytometry.Results： CKZ could inhibit cell apoptosis induced by H2O2、CHX and CTX.It also indicated that CKZ could upregulate NO production and multiple cytekine secretion by resting macrophages and downregulate NO release and cytokine synthesis on stimulated macrophages.Conclusion：CKZ could inhibit cell apoptosis.It also had dual-directional modulation of NO secretion and IL-6、IL-10、MCP-1、IFN-γ、TNF-α production.