中文摘要：

目的研究淫羊藿甙（ICA）对刀豆蛋白A（Con A）刺激的小鼠T淋巴细胞体外早期活化和增殖的影响。方法以MTT法检测T细胞药物毒性；利用流式细胞术（FCM）结合双色荧光抗体染色技术检测早期活化标志CD69分子的表达；运用流式细胞术结合活体染料羧基荧光素乙酰乙酸（CFDA-SE）染色技术和荧光抗体染色技术检测T细胞增殖。结果ICA在终浓度0．3、1．5、3．0μmol·L^-1时对于CD69的表达以及淋巴细胞48和72h增殖，均有明显的抑制作用（P〈0．01）。结论ICA能抑制Con A刺激的小鼠T淋巴细胞的早期活化和增殖，并呈剂量依赖性，即在最高浓度3．0μmol·L^-1时抑制率最强。

英文摘要：

Objective To study the effects of icariin （ICA） on the early activation and proliferation of routine T lymphocytes stimulated by concanavalin A （Con A） in vitro. Methods The chug toxicity of ICA on T lymphocytes was measured by MTT. Before stimulation with Con A （the final concentration was 5 mg·L^-1 ）, T ceils were treated with different concentrations of ieafiin for 4 h. The CD69 expression, the marker of early activation of T lymphoeytes, was measured by flow cytometry （FCM） combined with two-colour immunofluorescent staining of cell surface antigen. The proliferation of T lymphocytes was measured by flow cytometly combined with carboxyl fluorescin diaeetate succinimidyl ester （CFDA-SE） staining and immunofluorescent staining. Results ICA of 0. 3, 1.5, and 3.0 μmol·L^-1 inhibited the expression of CD69 （ P 〈 0. 01 ） and the proliferation of T cells （ P 〈 0.01 ）. Conclusion ICA significantly inhibits Con A-induced early activation and proliferation of mouse T lymphocytes in vitro, with a maximal inhibition in 3.0 μmol·L^-1 .