中文摘要：

为了分析米诺环素（MNC）对小鼠淋巴细胞体外活化和增殖的影响，探讨其免疫抑制作用机制．我们通过分离小鼠淋巴结细胞，以佛波醇酯（PDB）刺激，用不同终浓度的MNC与淋巴细胞共培养，荧光抗体染色结合流式细胞术，检测淋巴细胞早期和中期活化抗原CD69与CD25的表达；以CFSE染色，加入多克隆刺激剂佛波醇酯（PDB）和离子霉素（Ion）进行刺激，以流式细胞仪分析MNC对淋巴细胞增殖的影响；结果，荧光抗体染色显示MNC能显著抑制PDB诱导的T细胞CD69和CD25的表达；CFSE染色结果显示，MNC对佛波醇酯（PDB）和离子霉素（Ion）诱导48h的小鼠淋巴细胞增殖，具有明显的抑制作用（P〈0．05），且呈剂量依赖性．从而我们认为MNC对小鼠淋巴细胞的体外活化和增殖具有明显的抑制作用．具有独立于其抗菌活性的抗炎作用．

英文摘要：

To investigate the effect of MNC on activation and proliferation of the mouse T lymphocytes in vitro, in order to elucidate its immunosuppressive mechanism. Murine lymph node-derived lymphocytes were separated and stimulated with PDB and different experimental groups were set by co-cultured with MNC of different final concentration. Flow cytometry （FCM） was used to detect the activation （ the expressing of CD69 and CD25） and proliferation （CFSE staining） of lymphocytes. With the treatment of MNC, the expression rates of CD69 and CD25 in activated lymphocytes in response to PDB was reduced obviously; CFSE staining showed that at 48 h MNC could significantly inhibit the proliferation of mouse lymphocytes induced by PDB plus Ion In a dose-dependent manner （P 〈 0. 05）. Conclusively, MNC can effectively inhibit PDB stimulated activation and proliferation of murine lymphocytes, and has anti-inflammatory effects independent of its antimicrobial activity.