中文摘要：

目的研究咖啡酸苯乙酯（caffeic acid phenethyl ester．CAPE）对小鼠CD3^＋T淋巴细胞活化及增殖的影响，探讨其作用机制。方法无菌分离小鼠淋巴结淋巴细胞，制备淋巴细胞悬液。在淋巴细胞活化实验中，分别与不同浓度的咖啡酸苯乙酯预先孵育2h后，加入促分裂原伴刀豆蛋白A（ConA），运用荧光抗体染色技术结合流式细胞术。8h后。检测T淋巴细胞早期活化标记分子CD69的表达情况；24h后，检测T淋巴细胞中期活化标记分子CD25的表达情况；在淋巴细胞增殖试验中，运用CFDA-SE标记法检测T淋巴细胞刺激48h后的增殖情况。结果CAPE（0．5、1、5、10mg·L^-1）能够明显地抑制ConA刺激的小鼠淋巴细胞活化，且呈浓度依赖性；CAPE（0、5、1、5、10mg·L^-1）能够明显地抑制ConA刺激的小鼠淋巴细胞增殖，且呈浓度依赖性。结论CAPE对小鼠CD3^＋T淋巴细胞的体外活化和增殖具有抑制作用，其作用机制可能通过同时抑制PLC-γ信号途径和MAP激酶途径，由此推理CAPE是一种潜在的有效的免疫抑制剂。

英文摘要：

Aim To investigate the effects of caffeic acid phenethyl ester （CAPE） on activation and proliferation of murine CD3^＋ T lymphocytes, and to study the mechanisms of its immunomodulative effects. Methods Lymphocytes suspension was isolated and prepared sterilely from murine lymph nodes, and was incubated with different concentrations of CAPE for 2 h, then polyclonal activator Con A was added to activate the lymphocytes; double-fluorescence staining and flow cytometry were used to detect CD69 and CD25 expressions to evaluate activation level of CD3^＋ T lymphocyte, and CFDA-SE staining plus flow cytometry were used to analyze CD3^＋ T lymphocyte proliferation.Results CAPE （0.5, 1,5, 10mg· L^-1） could inhibit the expression of CD69 and CD25 of CD3^＋ T lymphocytes in a dose-dependent manner, and it also could inhibit T lymphocyte proliferation stimulated by Con A in a dose-dependent manner. Conclusion In vitro, CAPE could exert notably inhibition on activation and proliferation of murine CD3^＋ T lymphocytes, and its immunomodulative effects might result from affecting PLC-γ signaling and MAPK-signaling pathways. So CAPE was a potentially effective immunoinhibitory agent.