中文摘要：

目的研究人参皂苷Rg1对小鼠腹腔巨噬细胞的影响,并探讨其作用机制。方法无菌分离小鼠腹腔巨噬细胞,制备单细胞悬液,加入不同终浓度的人参皂苷Rg1 4 h后,细菌脂多糖LPS（10μg/ml）作用24 h,直径1μm的微球（1×1010/L）结合流式细胞术分析Rg1对小鼠腹腔巨噬细胞吞噬作用的影响;Griess试剂盒检测NO的释放;H2DCFDA染色检测ROS的含量;Fluo-4/AM染色检测Rg1对Ca2＋超载的影响;Sytox R Green染色,荧光酶标仪检测Rg1对CHX、CTX诱导的细胞凋亡的影响。结果 10、20μmol/L的Rg1时能明显抑制LPS诱导的巨噬细胞NO和ROS的产生以及吞噬微球的能力;10μmol/L的Rg1能显著抑制Ion诱导的的巨噬细胞Ca2＋的超载以及CHX、CTX诱导的细胞的凋亡。结论 Rg1具有显著的抗炎作用,并可抵抗多种因素诱导的细胞凋亡,为进一步开发为免疫治疗药物提供了依据。

英文摘要：

This study is designed to investigate the effects of ginsenoside Rg1 on murine peritoneal macrophages in vitro.The single cell suspension of murine peritoneal macrophages was prepared under sterile condition.After pre-incubated by Rg1 for 4 hours,the cells were stimulated with LPS at a final concentration of 10 μg/ml for 24 hours,then 1 μm diameter of the Yellow-Green Beads（1×1010/L） was added and the phagocytosis ability of macrophages was detected by flow cytometry（FCM）.At the same time,the amount of NO produced by macrophages was detected by Griess kit,the ROS production by macrophages was assayed by H2DCFDA staining,the intracellular calcium concentration in macrophages was deteced by Fluo-4/AM staining.SytoxR Green plus Fluorescence Microplate Rader were used to detect the cells survive condition.The results showed Rg1 could inhibit the phagocytosis ability of macrophage stimulated with or without LPS.It also indicated that Rg1 could inhibit the production of NO and ROS both in the presence and absence of LPS.In addition,Rg1 could reduce the apoptosis of peritoneal macrophages induced by ION.Furthermore,Rg1 could inhibit the cell apoptosis induced by CHX and CTX.All these prompt Rg1 has an significant anti-inflammatory effects on macrophages,and can reduce the apoptosis induced by different stimuli,which provides a basis for the further development of Rg1 as an immune therapy drug.