中文摘要：

目的利用α-半乳糖酰基鞘胺醇（α—galactosylceramide，α—Galcer）诱发性C57BL／6J小鼠流产模型，初步探讨NKT细胞活化与C57BL／6J小鼠流产的关系。方法孕7d和孕10dC57BL／6J小鼠，每组8只腹腔注射α—Galcer，72h后处死鼠剖腹探察胚胎数量。于0，24h取胎盘淋巴细胞，流式细胞术检测CD3^＋NK1．1^＋细胞百分率和CD69^＋／CD3^＋NK1．1^＋细胞百分率，ELISA方法检测α—Galcer处理不同时间血清孕酮（P）水平，并做子宫胎盘免疫组化切片观察。结果C57BL／6J小鼠可由α—Galcer引起流产。胎盘CD3^＋NK1．1^＋淋巴细胞在0，24h分别为（2．96±0．22）％和（4．17±0．67）％，CD69^＋／CD3^＋NK1．1^＋细胞百分率分别为（6．54±1．13）％和（82．45±5．85）％。ELISA方法测得血清P浓度为（0．15±0．01）ng／ml与对照组（1．28±0．03）ng／ml相比差异有显著性。胎盘子宫免疫组化切片显示α—Galcer处理组孕激素受体（PR）表达明显强于对照组。结论α—Galcer可诱发C57BL／6J小鼠流产，NKT细胞的活化及其分泌的细胞因子可能是导致流产的主要原因。

英文摘要：

Objective To develop a model of α - Galcer induced abortion in C57BL/6J mice and to investigate the relationship between natural killer T cell activation and abortion. Methods Pregnant C57BL/6J αmice were injected intraperitoneally with α - Galcer. Mice were sacrificed 72 hours later to exmamine the presence of abortion. Placental lymphocytes were collected at Oh and 24h. The ratio of CD3^＋ NK1.1^＋ and CD69^＋ / CD3^＋ NK1.1^＋ cells were determined with flow cytometr. Serum progesterone was determined by ELISA in mice receiving - Galcer treatment for different duration. The placenta was examined by immunohistochemistry. Results α - Galcer induced abortion in C57BL/6J. At Oh and 24th hour, the ratios of CD3^＋NK1.1^＋ lymphocytes in placenta was （2. 96±0. 22）% and （4. 17±0.67）%, and the ratios of CD69^＋/ CD3^＋NK1.1^＋ cells were （6. 54 ± 1.13）% and （82.45 ± 5. 85）%. Serum progesterone was significantly higher than that in the control. The expression of progesterone receptor in mice received α - Galcer administration was significant higher than that in the control. Conclusion α - Galcer induces miscarriage in C57BL/6J αmice, probably associ- ated with NKT activation and cytokine secretion.