中文摘要：

目的探讨漆黄素（fisetin）在体外对小鼠T淋巴细胞增殖、活化、凋亡、ROS的释放和巨噬细胞NO释放功能的影响。方法 CFDA-SE标记技术结合流式细胞术检测漆黄素对T淋巴细胞增殖及增殖指数（PI）的影响。荧光抗体染色结合流式细胞术分析漆黄素对T淋巴细胞在Con A的刺激下CD25的表达水平的影响。DIOC染色检测细胞线粒体膜电势的变化情况,H2DCFDA染色检测细胞ROS释放的变化。Griess试剂盒用以检测巨噬细胞NO释放功能。结果漆黄素（2.5,5和10μmol/L）明显抑制T淋巴细胞增殖,增殖指数（PI）降低并具有剂量依赖性。漆黄素可明显抑制CD25的表达（P〈0.01）及ROS的释放。DiOC6（3）染色分析显示,漆黄素可促进细胞的凋亡。巨噬细胞在LPS和IFN-γ的刺激下的NO释放量明显增加,而在漆黄素作用下NO释放量明显下降（P〈0.01）。结论漆黄素是一种潜在的免疫调节剂。

英文摘要：

This study aims to explore the effect of fisetin（FIS） extract on mouse T lymphocytes and the NO secretion of mouse macrophages further.We used Carboxyfluorescein diacetatesuccinimidyl ester（CFDA-SE） staining combined with flow cytometry assay to obtain the proliferation-related index（PI） of lymphocytes（72 h）.The expression level of CD25 of T lymphocytes stimulated with Con A was evaluated with flow cytometry.In order to detect the change of the mitochondrial membrane potential,cells were stained with 3,3-dihexyloxacarbocyanine iodide [DiOC6（3）].ROS production was monitored by flow cytometry using 2＇,7＇-dichlorodihydrofluorescein diacetate（H2DCFDA）.Griess kit was used to evaluate the NO production of macrophages.Our results showed that fisetin（2.5 μmol/L,5 μmol/L and 10 μmol/L） significantly inhibited the proliferation of T lymphocytes.At the same time fisetin could obviously inhibit the expression of CD25 and the accumulation of ROS.We also found that the apoptosis of the lymphocytes could be accelerated by fisetin in the presence of dexamethasone（DEX）.In addition,NO production of LPS plus IFN-γ-treated macrophages was clearly reduced by fisetin.These results indicate that fisetin might be a potential immunoregulation agent.