中文摘要：

目的通过深入研究AT-2灭活HIV-1颗粒对人外周血单个核细胞(peripheral blood mononuclear cells,PB-MCs)来源的CD4+T细胞中期活化标记分子(CD25)和晚期活化标记分子(CD71和HLA-DR)的作用,进一步探讨HIV病中普遍性的免疫过度活化的病理机制。方法AT-2灭活HIV-1IIIB型病毒颗粒,ELISA法检测p24抗原的含量,调整p24抗原含量为50 ng/mL;然后按照p24抗原量为0.1 ng/mL(记作HIV-1 1/500组)、1 ng/mL(记作HIV-1 1/50组)和10 ng/mL(记作HIV-11/5组)的浓度加入到PBMCs中,以植物血凝素(phytohemagglutinin,PHA)组为阳性对照,24 h后,运用免疫荧光抗体染色技术结合流式细胞术检测PBMCs中CD4+T细胞CD25的表达百分率;48 h后,运用免疫荧光抗体染色技术结合流式细胞术检测PBMCs中CD4+T细胞CD71和HLA-DR的表达百分率。结果24 h后,空白对照组中,CD4+T细胞CD25的表达百分率为(9.46±2.12)%,PHA组为(79.45±3.72)...

英文摘要：

Objective To explore the pathological mechanisms of immune overactivation in HIV disease by investigating the effects of AT-2-inactivated HIV-1 particles on expression of CD25,CD71 and HLA-DR on CD4+T cells from human peripheral blood mononuclear cells(PBMCs).Methods HIV-1IIIB particles were inactivated by AT-2 and the concentration of p24 antigen was quantitated by p24 ELISA,then the AT-2-inactivated HIV-1IIIB particles were added to human PBMCs culture system in serial concentrations to stimulate cells,wi...