中文摘要：

分别采用MTT法、CFSE染色结合流式细胞术、双色荧光抗体染色结合流式细胞术、PI染色法检测去铁胺（DFO）对小鼠T淋巴细胞的药物毒性、对T淋巴细胞增殖、对ConA刺激下的小鼠T细胞CD69表达和淋巴细胞周期的影响。研究结果表明：DFO对淋巴细胞的毒性T细胞体外增殖、活化和细胞周期无影响；不同浓度的DFO（5、10、20、40μmol脚能显著抑制受ConA刺激48h和72h后的T淋巴细胞增殖；低浓度的DFO（5、10μmol／L）对小鼠T淋巴细胞CD69的表达没有影响，高浓度（20、40μmol／L）的DFO可抑制T淋巴细胞CD69的表达：Ph能将T淋巴细胞抑制在G0/G1期。说明DFO除可通过抑制T淋巴细胞活化外，可能还通过其他机制抑制T淋巴细胞增殖，且DFO可以将淋巴细胞周期抑制在G0/G1期，可望发展成为一种新的免疫抑制干预药物。

英文摘要：

In this paper, the cytotoxiciy of DFO to lymphocytes was evaluated using MTT assay, carboxyfluorescein diacetatesuccinimidyl ester （CFDA-SE） staining plus flow cytometry assay was employed to investigate the effect of DFO on the proliferation of the lymphocytes, the expression levels of CD69 on T lymphocytes stimulated by Con A were evaluated with flow cytometry after staining with fluorescent monoclonal antibody, and Cell-cycle distribution of T lymphocytes was also analyzed by propidium iodide staining. The results showed that DFO induced-cytotoxicity had little impact on the reliability of our experiment data; DFO （5,10,20,40 μmol/L） significantly inhibited the proliferation of T lymphocytes stimulated by Con A for 48 h and 72 h;DFO at low concentrations （5,10 μmol/L ） exerted no influence on the expression of CD69, however, DFO at high concentration （20, 40 μmol/L ） could inhibit the expression of CD69. The cell cycle distribution analysis revealed that DFO could induce a cell cycle arrest in G0/G1 phase. We can draw conclusion that DFO may use other mechanism except the inhibition of activation of T lymphocyte, to inhibit the proliferation of lymphocyte, it can induce a cell cycle arrest in G0/G1 phase. This effect demonstrates that DFO has the potential to be developed into a new immuo-intervention drug.