中文摘要：

本研究针对天然交联剂原花青素处理牛心包材料的性能进行研究。采用原花青素交联牛心包组织,制备人工生物心脏瓣膜材料,并对其交联特性、力学特性、抗酶降解性能、亲疏水性能、细胞毒性试验以及溶血试验等进行分析。结果显示：原花青素或戊二醛处理的牛心包组织变性温度、力学特性、表面亲水性能和抗酶降解能力都明显提高;与传统交联剂戊二醛相比组织稳定性、亲水性能和抗酶降解能力未见显著性差异,但是最大断裂强度显著提高（原花青素交联组织最大断裂强度（13.863 0 MPa）明显高于戊二醛交联组织（10.784 2 MPa）;溶血试验结果表明原花青素处理固定组织的溶血率（2.61%）远低于戊二醛处理的组织（12.54%）;细胞毒性试验结果表明原花青素交联组织在1、3、5 d的细胞增殖率分别为76.19%、88.96%、100.12%,而戊二醛在相同的时间点的细胞增殖率分别为63.15%、57.28%、48.74%。原花青素交联的瓣膜材料组织结构稳定、亲水性好、毒性小且能维持较好的力学性能,作为人工生物瓣膜材料具有很好的研究前景。

英文摘要：

The aim of this study is to evaluate the crosslinking effect of procyanidins,a kind of natural crosslinking reagent,on the materials of bioprosthetic valve.After fixing bovine pericardiac tissues by procyanidins,crosslinkng characteristics,mechanical properties,the in vitro enzymatic degradation resistance,the hydrophilicity,the vitro cytotoxicity test and hemolysis tests were performed.For fixation of biological tissue with glutaraldehyde or procyanidins,there were increase in denaturation temperature,the surface hydrophilicity and mechanical properties as well as in vitro enzymatic degradation resistance.There were no significant differences in denaturation temperature,mechanical properties,the hydrophilicity and the in vitro enzymatic degradation between the glutaraldehyde and procyanidins fixed tissues.However,the ultimate tensile strength of the procyanidins fixed tissues（13.863 0 MPa） were significantly superior to that of the glutaraldehyde fixed tissues（10.784 2 MPa）.The hemolysis tests showed that hemolysis rate of the procyanidins fixed tissues（2.61%） was lower than that of the glutaraldehyde fixed tissues（12.54%）.The vitro cytotoxicity test showed that at 1,3,5 d the cell relative proliferation rate of the tissue fixed by procyanidins was 76.19%,88.96%,100.12%,respectively,and the cell relative proliferation rate of the glutaraldehyde-fixed tissue was 63.15%,57.28%,48.74%,respectively.This results revealed that procyanidins could crosslink bovine pericardia effectively without toxicity compared with glutaraldehyde.Our results suggestes that this method might be a useful approach for the preparation of bioprosthetic heart valve.