中文摘要：

这个工作检验了最佳的 syringing 深度在期间在 vitro 房间装载以便在 syringing 以后弄平房间分发在柱体的一滴房间暂停 poly (lactide-co-glycolide )(PLGA ) 多孔的脚手架。10 公里高度和 10 公里直径的脚手架基于球形的 porogens 经由塑造的房间温度压缩与微粒沥滤技术被制作。在 vitro，测试为如此的考试被采用：3-(4,5-dimethylthiazol-2-yl ) 染色的装载房间的脚手架的全球观察 -2,5-diphenyltetrazolium 溴化物(MTT ) 技术和在切装载房间的脚手架进层以后的房间的空间分发的量的大小。仅当起始的房间暂停在在最高的表面下面，但是在脚手架的中间上面的层上被播种，房间的平分布很快被完成，这被发现。可获得性在在如此的一种球形毛孔的 PLGA 的老鼠骨头髓干细胞的 vitro osteoblastic 区别，脚手架同时被证实。

英文摘要：

This work examined the optimal syringing depth during in vitro cell loading in order to even cell distribution after syringing a drop of cell suspension in cylinder poly（lactide-co-glycolide） （PLGA） porous scaffolds. The scaffolds of 10 mm height and 10 mm diameter were fabricated via room-temperature compression molding ＆ particulate leaching technique based on spherical porogens. In vitro tests were employed for such examinations： a global observation of a cell-loaded scaffold stained by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） technique and a quantitative measurement of spatial distribution of cells after slicing the cell-loaded scaffolds into layers. It was found that an even distribution of cells was soon achieved only if the initial cell suspension was seeded on the layer that was below the top surface but above the middle of scaffolds. The availability of in vitro osteoblastic differentiation of rat bone marrow stem cells in such a kind of spherical-pore PLGA scaffolds was meanwhile confirmed.