中文摘要：

这研究的目的是评估 crosslinking 效果一自然地 bioprosthetic 心阀门的材料上的 crosslinking reagent-procyanidins (PA ) 。在由 procyanidins 修理牛的心囊的纸巾以后， crosslikng 特征，机械性质，在 vitro，酶的降级抵抗， hydrophilicity 和溶血测试被检验。结果证明有 glutaraldehyde (GA ) 或 procyanidins 的生物织物的固定象在 vitro 一样增加了它的变性作用温度，表面 hydrophilicity 和机械性质酶的降级抵抗。在变性作用温度没有重要差别，机械性质， hydrophilicity 并且在里面在 glutaraldehyde 和 procyanidins 之间的酶的降级修理了的 vitro 纸巾。然而， procyanidins 的最终的张力的力量修理了纸巾比 glutaraldehyde 显著地优异固定纸巾。溶血测试证明 procyanidins 的那溶血率修理了纸巾比 glutaraldehyde 的修理纸巾的低。这研究显示出那个 procyanidins 罐头交叉连接它牛的 pericardiaa 有效地没有毒性。我们的结果建议这个方法可能是为 bioprosthetic 心阀门的准备的一条有用途径。

英文摘要：

The aim of this study was to evaluate the crosslinking effect of a naturally crosslinking reagent-procyanidins （PA）-on the materials of bioprosthetic heart valves. After fixing bovine pericardial tissues by procyanidins, crosslikng characteristics, mechanical properties, in vitro enzymatic degradation resistance, the hydrophilicity and hemolysis tests were examined. The results showed that the fixation of biological tissue with glutaraldehyde （GA） or procyanidins increased its denaturation temperature, the surface hydrophilieity and mechanical properties as well as in vitro enzymatic degradation resistance. There were no significant differences in denaturation temperature, mechanical properties, the hydrophilicity and the in vitro enzymatic degradation between the glutaraldehyde and procyanidins fixed tissues. However, the ultimate tensile strength of the procyanidins fixed tissues was significantly superior to the glutaraldehyde fixed tissues. The hemolysis tests showed that hemolysis rate of the proeyanidins fixed tissues was lower than that of the glutaraldehyde fixed tissues. This study shows that procyanidins can crosslink which bovine pericardiaa effectively without toxicity. Our results suggest that this method might be a useful approach for the preparation of bioprosthetic heart valve.