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Preparation of a gene-engineering mutant of bacteriorhodopsin BR-D96V and corresponding poly（vinyl alcohol）-based functional composite films

ISSN号：1008-6455
期刊名称：《中国美容医学》
时间：0
分类：S511[农业科学—作物学] Q63[生物学—生物物理学]
作者机构：[1]Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, China, [2]Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China, [3]Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China
相关基金：We thank Professor Norbert A. Hammp in Marburg University for provid- ing mutant BR-D96N and Professor Richard Needleman in Wayne State University for providing plasmid pNov-R-mi-ni and Halobacterium sali- narum strain L33. This work was supported by the National Basic Re- search Program of China （2009CB930000） and National Natural Science Foundation of China （50533010）.

作者： ZHAO YingChun[1,2], MING Ming[1], HONG Jie[2], MA DeWang[1], WU Jia[1], LI QingGuo[3], HUANG WeiDa[2], DING JianDong[1]

关键词：细菌视紫红质, 聚乙烯醇, 基因工程, 突变体, 复合膜, BR, 制备, 聚合物基体, synthetic polymer, organic photoelectric material, composite film, bacteriorhodopsin, site-specific mutagenesis, photoresponsive protein

中文摘要：

Bacteriorhodopsin (BR ) 展出，作为在象功能的信息材料的 Halobacterium salinarum，唯一的 photoresponsive 行为，和表演诺言的膜蛋白质。有缬氨酸(BR-D96V ) 代替的第 96 丁氨二酸酸的 BR 的新异种被获得然后在一个合成聚合物矩阵的 BR-D96V 的一部合成电影在这研究被准备。变异的 BR-D96V 在 bacterio 视蛋白被表示由基因工程的缺乏的 halobacterial 紧张(L33 ) 。尽管缬氨酸是很恐水病的，也就是，这点异种保留基本生物活动光电并且对光反应变色回答。不过，在 BR 异种中间的 M 的一生比在中立水的答案的野类型的 BR 的长将近二个数量级，它作为信息材料有益于它的潜在的应用程序。M 一生进一步显著地在把 BR-D96V 嵌进以后被延长 poly (乙烯基白酒)(PVA ) 。BR-D96V 是很敏感的与野类型的 BR 和另一 BR 异种比较浇内容，这也被发现。

英文摘要：

Bacteriorhodopsin （BR） exhibits, as a membrane protein in Halobacterium salinarum, unique photoresponsive behaviors, and shows promise as a functional information material. A new mutant of BR with the 96th aspartic acid replaced by valine （BR-D96V） was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this research. The mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain （L33） by gene engineering. Although valine is very hydrophobic, this point mutant keeps the basic biological activities, namely, photoelectric and photochromic responses. Nevertheless, the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution, which benefits its potential application as an information material. The M lifetime is further signifi- cantly prolonged after embedding BR-D96V into poly（vinyl alcohol）（PVA）. It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.

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