东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

Effects of L-lactic acid and D,L-lactic acid on viability and osteogenic differentiation of mesenchymal stem cells

ISSN号：1000-3304
期刊名称：《高分子学报》
时间：0
分类：TQ921.3[轻工技术与工程—发酵工程;化学工程] Q813.11[生物学—生物工程]
作者机构：[1]State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Advanced Materials Laboratory, FudanUniversity, Shanghai 200433, China
相关基金：supported by the National Basic Research Program of China (2009CB930000, 2011CB606203);the National Natural Science Foundation of China (21034002, 91127028, 51273046)

关键词：骨髓间充质干细胞, L-乳酸, 成骨分化, 细胞毒作用, 能力, 细胞毒性, 可生物降解, 生物相容性, biodegradable polymer, biomaterial, tissue engineering, lactic acid, cytotoxicity, biocompatibility, mesenchymal stem cells

中文摘要：

Poly (乳的酸)(PLA ) 并且包含乳的酸的单位的另外的脂肪族的聚酯是很流行的可被细菌破坏的材料。当时降级产品，乳的酸，担忧与对 biocompatibility 的否定影响带，集中的试验性的研究少些被报导。这研究针对一在里面 L 乳的酸和 D 的 cytotoxicity 的 vitro 检查， L 乳的酸。从老鼠骨头髓导出的间充质的干细胞(MSC ) 被采用测试乳的酸的 cytotoxicity。认为乳的酸的增加不仅介绍喂奶组织而且改变中等 pH 和离子力量，这三候选人效果被设置不同比较组以一个 decoupled 方法检验。结果证实中等 pH 的变化是占优势的因素。D 喂奶是比在高集中的 L 喂奶更细胞毒素的，这也被发现了。然而， L 乳的任何一个或 D， L 乳的酸在大多数医药应用似乎可接受，因为 cytotoxicity 仅仅是重要的当集中象为他们俩的 20 mmol/L 一样高时。

英文摘要：

Poly（lactic acid）（PLA） and other aliphatic polyesters containing the unit of lactic acid are very popular biodegradable materials. While the degradation products, lactic acids, have been worded to bring with negative influence on biocompatibility, the focused experimental studies are less reported. This study is aimed at an in vitro examination of cytotoxicity of both L-lactic acid and D,L-lactic acid. Mesenchymal stem cells （MSCs） derived from rat bone marrow are employed to test the cytotoxicity of the lactic acids. Considering that the addition of lactic acids not only introduces lactate groups but also alters medium pH and ion strength, these three candidate effects are examined in a decoupled way by setting different comparison groups. The results confirm that the change of medium pH is the predominant factor. It has also been found that D-lactate is more cytotoxic than L-lactate at high concentrations. Yet, either L- or D,L-lactic acids seem acceptable in most of medical applications, because the cytotoxicity is significant only when the concentrations are as high as 20 mmol/L for both of them.