位置:成果数据库 > 期刊 > 期刊详情页
Cell orientation on a stripe-micropatterned surface
  • ISSN号:1008-6455
  • 期刊名称:《中国美容医学》
  • 时间:0
  • 分类:O632.31[理学—高分子化学;理学—化学] Q813.11[生物学—生物工程]
  • 作者机构:[1]Key Laboratory of Molecular Engineering Polymers, Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, China
  • 相关基金:Supported by the National Basic Research Program(Grant No. 2009CB930000), Key Project of the Ministry of Education (Grant No. 305004) and Science and Technology Developing Foundation of Shanghai (Grant No. 07JC14005)
中文摘要:

Stripe-micropatterned 表面最近是一个唯一的工具学习房间取向。在这篇论文,我们准备了,由影印石版术转移技术,稳定的黄金(Au ) 木钉水疗院胶化表面与上的微模式定义房间抵抗(木钉水疗院胶化) 并且房间粘合剂(金微条纹) 性质。3T3 成纤维细胞在 Au-microstripe 表面上是有教养的观察房间粘附和取向。五个统计参数被定义并且过去常在微模式上描述房间取向。随内部条纹的距离的增加, orientational 顺序参数,一个房间的长、短的斧子的比率,和条纹上的房间的职业部分逐渐地增加了,而一个单个房间的传播区域减少了。这四个参数的突然的变化没发生在一样内部距离。在散布区域的房间上的 Au 条纹上的一个房间的粘附比率没作为内部条纹的距离的功能 monotonically 变化。5 个统计参数的联合代表了房间取向行为半份量上很好。

英文摘要:

Stripe-micropatterned surfaces have recently been a unique tool to study cell orientation. In this paper, we prepared, by the photolithography transfer technique, stable gold (Au) micropatterns on PEG hydrogel surfaces with defined cell-resistant (PEG hydrogel) and cell-adhesive (gold microstripes) properties. 3T3 fibroblasts were cultured on Au-microstripe surfaces to observe cell adhesion and orientation. Five statistical parameters were defined and used to describe cell orientation on micropatterns. With the increase of inter-stripe distance, the orientational order parameter, the ratio of long and short axes of a cell, and the occupation fraction of cells on stripes increased gradually, whereas the spreading area of a single cell decreased. The abrupt changes of these four parameters did not happen at the same inter-distance. The adhesion ratio of a cell on Au stripes over cell spreading area did not change monotonically as a function of inter-stripe distance. The combination of the 5 statistical parameters represented well the cell orientation behaviors semi-quantitatively.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《中国美容医学》
  • 主管单位:教育部
  • 主办单位:西安交通大学 第四军医大学
  • 主编:朱宏亮
  • 地址:西安市新科路1号东兴科技大厦12层
  • 邮编:710043
  • 邮箱:zgmryx@163.com yishi010@163.com
  • 电话:029-82218513 82251091-8833
  • 国际标准刊号:ISSN:1008-6455
  • 国内统一刊号:ISSN:61-1347/R
  • 邮发代号:52-27
  • 获奖情况:
  • 中国科技论文统计源期刊,中国学术期刊综合评价数据库来源期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版)
  • 被引量:28344