中文摘要：

目的现在较为广泛应用于临床的人工韧带[聚对苯二甲酸乙二醇脂（PET）]——因为所固有的疏水性，使其在临床应用受到一定局限，本实验拟寻找促进材料与骨的相容的新方法。方法运用临床常用的涂层技术，以生物玻璃（58S）作为涂层材料作用于PET纤维表面，将成骨细胞（MC3T3-E1）培养在以涂层PET纤维和未涂层PET纤维上，观察成骨细胞的细胞增殖能力和细胞活性。结果细胞计数的第1、3、5天，细胞数量在涂层组与空白组均呈现增长趋势，但是在5d涂层处理组明显高于未处理组[（6．25±0．27）×104比（8．92±0．17）×104，P〈0．05]，而在噻唑蓝法（MTT）和碱性磷酸酶（ALP）活性测定中，涂层组在第1、3、5天均表现出明显的优势（P〈0．01）。结论经生物玻璃涂层处理的PET纤维组能显著提高成骨细胞的增殖及生物学活性。

英文摘要：

Objective To search for a novel method to promote the compabibility of materials and bone. The anterior cruciate ligament （ACL） reconstruction using artificial ligament [ polyethylene tereph- thalates （PET）] , which has good biocompatibility and mechanical properties, is prevalent. However, the low tendon-to-bone healing rate due to the PET＇ s hydrophobicity limits the clinical application. Methods This study aimed to observe the effects of the coating bioactive glass （58S） synthetic anterior eruciate liga- ment （ACL） on osteoblastie proliferation and eytoaetivity in vitro. By using coating technique, bioaetive glass （58S） was coated on the polyethylene terephthalates （PET） fibers. The scanning electron micro- graphs showed that bioaetive glass （58S） were spindle-shaped and well-orientated on the PET fibers. Then, MC3T3-E1 osteoblasts were seeded onto the PET or the coating PET. Results The number of osteo- blasts was significantly increased in the coating PET （8.92 ± 0. 17 ） as compared with the PET （6. 25± 0. 27 ） by using automated cell counter at the 5th day （P 〈 0. 05 ）. Alkaline phosphatase activity and meth- yl thiazol tetrazolium （MTF） test also showed that the coating fibrous scaffold induced significnat osteogen- esis and cell proliferation as compared to PET without coating. Conclusion The coating bioaetive glass （58S） provides an instructive microenvironment for osteoblast proliferation.