中文摘要：

目的观察经生物玻璃（58s）涂层处理的聚对苯二甲酸乙二醇脂（Polyethylene terephthalates，PET）对体外及体内移植物与骨愈合的影响。方法58S与明胶以质量比1：4混合，制备PET涂层材料（58S—PET），对照为不经涂层处理的PET材料。将4×10^4／ml成骨细胞系（MT3T3-E1）培养于24孔板的两种不同材料中，培养后1、3、5天进行四唑盐比色法及碱性磷酸酶测定。将24只新西兰大白兔随机分为两组：58S-PET组和PET组，每组12只，建立半关节腱-骨愈合模型。术后6、12周进行组织形态学及生物力学测试，观察移植物与宿主骨的愈合。结果在四唑盐比色法和碱性磷酸酶活性测定中，58S-PET组吸光度在第3、5天均表现出明显的优势。两种材料植入动物半关节腱-骨愈合模型后，随时间推移最大拔出力均有明显提高。58S—PET组6周最大拔出力（61．70±6．95）N，高于PET组（45．21±9．78）N；58S-PET组12周最大拔出力（89．25±9．50）N，高于PET组（71．38±6．26）N。两组差异均有统计学意义。术后6、12周，组织学观察到两组材料与宿主骨的骨界面模糊，有新生骨形成，以58S—PET组更为明显。结论经生物玻璃涂层处理的PET材料能提高成骨细胞的增殖和活性，促进新生骨生成，缩短移植物与骨的愈合过程。

英文摘要：

Objective To observe the effect of polyethylene terephthalates （PET） coated with 58S bioactive glass on graft-bone healing. Methods The PET coated with 58S hioactive glass was used in experimental group, and uncoated PET was used as a control. The coating solution was made of 20% bioaetive glass powder and 80% gelatin powder （by weight）. In our vitro study, 4×10^4/m] MT3T3-E1 cells were cultured in 24-well plates with the coated or uncoated PET, and the MTT and ALP were tested at 1, 3, 5 days to show the proliferation and the activity of the cells. The SEM and the X-ray photoelectron spectrometer were adopted to analyze the surface characteristics of the fiber. In our vivo study, 24 skeletally mature New Zealand white rabbits were randomly divided into two groups, the 58S-PET group and the PET group. Both groups underwent a surgical procedure to establish a tibia-articular tendon-bone healing model. Mechanical examination and histological assay were taken to verify the coating effect in vivo. Results The 58S-PET group showed significantly differences in both the MTF and ALP tests at each time point （3, 5 days） compared with the PET group. In the animal experiments, the maximum load inereased by time in both groups. At 6 weeks, the load-to-failure was significantly higher in the 58S-PET group [（61.70±6.95） N] than that of the PET group [（45.21±9.78） N]. At 12 weeks, the load-to-failure was also significantly higher in the 58S-PET group [（89.25±9.50） N] than that of the PET group [（71.38±6.26） N]. In the histological assay, it was found that there was new bone formation in the indistinct interface between the graft and the host bone in both groups at 6, 12 weeks, and a stronger binding was seen in the 58S-PET group than in the PET group. Conclusion The 58S-PET could enhance the proliferation and activity of the osteoblast and therefore promote the new bone formation and subsequently leads to a positive effect on tendon-bone healing.