中文摘要：

目的探讨不同抗凝剂和温度对红细胞渗透脆性试验结果的影响。方法40例肝素锂抗凝血分别在16℃、23℃和37℃下进行红细胞渗透脆性试验；另取40例新鲜血液分别用EDTA—K2、枸橼酸钠（ACD）、肝素锂在37℃下进行测定，比较不同试验条件下溶血率的差异。各组以肝素锂抗凝血在37℃测定结果为对照，分为低脆性组和正常脆性组加以比较。结果16℃、23℃和37℃组脆性结果比较，正常脆性组在23℃的溶血率结果差异无统计学意义（P〉0．05），其他各样本组的溶血率差异均有统计学意义（P〈0．01），其中低脆性组差异最为显著，23℃和16℃的结果分别平均偏高28．7％和59．7％；ACD、EDTA-K2与肝素锂抗凝血红细胞脆性结果比较，各样本组差异均有统计学意义（P〈0．01），其中低脆性组差异最为显著，分别平均偏高16．7％和37．7％。结论不同抗凝剂和温度对红细胞渗透脆性影响较大，应使用试剂盒所规定使用的肝素类抗凝剂而不宜使用EDTA—K2和ACD抗凝，并应严格控制在37℃恒温水浴条件下测定。

英文摘要：

Objective To discuss influences on erythrocyte osmotic fragility （one tube test） result with different anticoagulants and temperatures. Methods In order to evaluate the differences of the hemolysis rate under different conditions,40 anticoagulative blood samples with lithium-heparin were tested for red cell osmotic fragility test at 16 ℃, 23 ℃, 37 ℃ ,at the same time,and 40 ED TA-K2 ,sodium citrate and lithium-heparin treated fresh blood samples for controlled were tested at 37 ℃. Each group was divided into low brittle group and normal brittle group,with anticoagulative blood samples with lithium-heparin served as control. Results Compared with 16 ℃ and 37 ℃ group,the hemolysis rate of normal brittle group at 23℃ has no significant difference（P〉0.05）, but other groups all have significant difference（P〈0. 01）,especially the low brittle group. The average of 23 ℃ and 16 ℃ group were higher 28.7% and 59.7% respectively. Each group has significant difference（P〈0.01） compared with sodium citrate,EDTA K2 and lithium-heparin treating blood groups,especially the low brittle group. The average of the low brittle group was higher 16. 7% and 37.7% respectively. Conclusion Different anticoagulants and temperatures have great influence on erythrocyte osmotic fragility tests. Neither EDTA-K2 nor sodium citrate should be used,but anticoagulant such as lithium-heparin provided from the kit. And the tests should be carried out at 37℃ in constant water hath strictly.