中文摘要：

目的本研究的目的是研究由南京脉迪森医药科技有限公司生产的仿松质骨生物活性人工骨（磷酸氢钙／胶原复合人工骨）诱导大段骨缺损的动态过程。方法使用5只新西兰大白兔作为实验动物，所有动物被戊巴比妥钠溶液局部麻醉后在一侧尺骨造成1cm全缺损．在缺损处植入上述人工骨。在术后立即及0、7、14、21、35、42、49、55、62、69、76天拍X线照片，最后处死动物进行解剖观察及组织学观察。结果X线照片显示术后7天可见缺损处骨组织大量增生，至术后49天可见缺损处合拢但尚未完全愈合，术后49至55天出现明显的过度增生及与桡骨粘连，此后经历增生与吸收的过程直至完全愈合，术后76天可见完全愈合。处死动物进行解剖可见与桡骨粘连处已被吸收，缺损处修复与未手术部位外观接近。组织切片可见缺损处形成骨板并完全骨化、骨单位明显、尚未形成明显的层状骨板，散布的骨窝中有骨细胞、中央管可见新生血管，未见人工骨残留，人工骨被彻底降解吸收。结论本实验表明南京脉迪森医药科技有限公司生产的仿松质骨生物活性人工骨可替代自体骨移植修复大段骨缺损．植入后3个月内即诱导自体骨再生而使缺损部位完全复原，是一种性能优异的骨修复材料。

英文摘要：

Objective The present study was aimed to observe the dynamic rehabilitation process of rabbits＇ massive bone defects by calcium hydrogen phosphate/collagen composites produced by Nanjing Medicine Sci. ＆ Tech.. Co., Ltd.. Methods Five New-Zealand rabbits were used as experimental animals for composites implantation. After anesthetized with pentobarbital sodium, every rabbit got a 10 mm bone defect at left ulna. The calcium hydrogen phosphate/collagen （DCP/CO） bone substitutes were inserted into defects. Biocompatibility and osteoinductive potentials of these bone substites were assessed with macroscopic examination, histological examination, dynamic rehabilitation process was observed by X-ray photography at 0, 7, 14, 21, 35, 42, 49, 55, 62, 69, 76 days after implantation in vivo. Resutls Calcium hydrogen phosphate/collagen （DCP/CO） composites were completely biodegraded and absorbed within 76 days after surgery, the defects were completely repaired. X-ray photography demanstrated that ossification and bone lamella at defects, osteo-regenesis could be observed 7 days after implantation, the two ends of defects closded up 49 days after implantation, hyperplasia and adhesion with radius occurred between 49 to 55 days after implantation. Then underwent hyperplasia and absorption until healed completely 76 days after implantation. Histological examination demanstrated that osteon was obvious, but bone lamellar structure was not obvious. Osteoblasts could be seen in dispersed bone lacunas. There were vessels in bone central canals. No compostie remnants were observed. Conclusion Calcium hydrogen phosphate/collagen composites imitating cancellous bone produced by Nanjing Medicine Sci. ＆ Tech. Co., Ltd. could be used to repair massive defect in place of autogeneous bone graft.