This paper took Chishui River Basin as the research object,and pointed out that its culture,which reflected local life and production and was featured with regional characteristics as well as abundant cultural connotation,was constituted by native natural and cultural geography,folk customs and historical feature.The relation between Chishui salt path and salt business culture under the typical closed and half-closed geographical environment was analyzed to indicate that the realignment of Chishui River had brought prosperity of Chishui River,booming of commercial towns,and development of ancient architectures and guild hall culture.The relation between rich natural resources and production and life culture of Chishui River was explained to show that Danxia landform nurtured stone culture,Chishui River resources cultivated fishing culture,special hydrogeological environment fostered liquor culture represented by Maotai,and Bamboo culture accumulated for hundreds of years.Finally,it introduced military culture and the spirit of the Long March forming based on the special location of Chishui River.It emphasized that geological environment was the important basis for human’s survival and played an immeasurable role in the cultural development of a region.