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野生毛花猕猴桃雄株居群花粉形态观察
  • ISSN号:1009-9980
  • 期刊名称:《果树学报》
  • 时间:0
  • 分类:S663.4[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]江西农业大学林学院,南昌330045, [2]江西农业大学农学院,南昌330045
  • 相关基金:国家自然科学基金(31360472);国家科技基础性工作专项(2012FY110100-7);江西省重大科技专项(20143ACF60015);江西省现代农业产业技术体系建设专项(JXARS-05);研究生创新专项基金(NDYC2015-B002)
中文摘要:

【目的】分析江西省麻姑山自然保护区内野生毛花猕猴桃花粉的形态特征,了解其遗传变异性、亲缘关系与演变规律。【方法】利用扫描电子显微镜对23个野生毛花猕猴桃雄株的花粉粒进行了形态观察。【结果】毛花猕猴桃雄株花粉粒外观为长球形,偶有近超长球形;极面观为三裂圆形,极轴长22.42~27.73μm,变异系数为4.75%;赤道轴长12.73~14.31μm,变异系数为3.12%。具3萌发沟,赤道面可见1~2条,等间距分布,萌发沟长20.52~24.68μm,变异系数为5.11%;萌发沟脊宽7.29~9.52μm,变异系数为6.78%;根据NPC分类系统分类,属于N3P4C5型花粉。花粉外壁纹饰有3种类型:脑纹状、波纹状和疣状纹饰,分别占花粉样品的65.22%、30.43%和4.35%。聚类分析结果显示,多数雄株亲缘关系较近。【结论】该居群内野生毛花猕猴桃雄株花粉的形态有较多的共性特征,同时在花粉的形态、外壁纹饰上,不同单株间存在差异,分别呈现毛花猕猴桃的遗传保守性和多样性。花粉外壁纹饰的演化趋势为:由波纹状向疣状和脑纹状演化。

英文摘要:

[Objective] The shape and the exine sculpture could be used for examining plant evolution and genetic relationship. The objective of the study was to explore genetic diversity, relationship among naturally exiting male population of Actinidia eriantha Benth. [ Methods ] Fresh pollen grains were collect- ed from mature staments before anthesis. The specimens came from A. eriantha Benth. male geotypes growing in the Magu Mountain region in Naneheng county, Fuzhou city, Jiangxi province, southeast of Chi- na. Morphological characteristics of the materials were typical and representative. Pollens were directly placed on conducting resin without any treatment, coated with gold using a Cressington 108 sputter coater and observed with a Quanta 250 scanning electron microscope (SEM). 15-20 pollen grains were measured for each male geotype. The equatorial and polar faces were studied at 5 000 x magnification. The exine sculptures were studied at 15 000x magnification. Five characters were measured: P (polar diameter), E (equatorial diameter), D (colpus length), W(colpus width), and P/E ratio. Data Processing System was used to analyze the data. [ Results ] The shapes of pollen grain of wild A. eriantha male geotypes were prolate or perprolate, the polar view were trifid circular. The pollen polar axis lengths ranged from 22.42 to 27.73 p,m, the coefficient of variation was 4.75%. The equatorial axis length ranged from 12.73 to 14.31 μm, the coefficient of variation was 3.12%. All pollen grains were tricolporate including three ectocolpi and three endopori. Ectoeolpi was long but narrow at two ends and arranged in a regular lontiudinal pattern. One or two colpi were observed from equatorial view. Three colpi were found from polar view. Colpus widths ranged from 7.29-9.52 μm, the coefficient of variation was 6.78%. There were significant differences be- tween individual geotypes. According to the NPC(Number, Position, Character) classification system clas- sification, the pollens belonged to N3P4

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期刊信息
  • 《果树学报》
  • 北大核心期刊(2011版)
  • 主管单位:农业部
  • 主办单位:中国农业科学院郑州果树研究所
  • 主编:王志强
  • 地址:河南省郑州市航海东路南中国农业科学院郑州果树研究所
  • 邮编:450009
  • 邮箱:chinagsxb@163.con
  • 电话:0371-65330928 653387308
  • 国际标准刊号:ISSN:1009-9980
  • 国内统一刊号:ISSN:41-1308/S
  • 邮发代号:36-93
  • 获奖情况:
  • 获河南省优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:18699