中文摘要：

目的：研究胞内钙离子释放阻断剂8-（N,N-二乙胺）辛基-3,4,5-三甲氧基苯甲酸酯（TMB-8）对刀豆蛋白A（ConA）介导的小鼠T淋巴细胞体外活化、增殖和细胞周期的影响。方法：以ConA作为T细胞活化、增殖的刺激剂,以不同浓度的TMB-8及与环孢菌素A（CsA）联合作用于该系统,用流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸（CFDA-SE）染色流式细胞术,分析TMB-8在ConA刺激下小鼠淋巴细胞的增殖相关指数（PI）;以碘化丙啶染色分析细胞周期的分布情况。结果：ConA作用6h后,CD69＋T细胞的比率为（74.88±1.88）%,TMB-8在终浓度10、20、40μmol/L下均抑制ConA介导的T细胞CD69表达（P〈0.01）,其中,40μmol/L的TMB-8为（52.55±1.54）%,达到最高抑制率。培养48h和72h,ConA刺激下的PI值分别为1.24±0.01和2.05±0.07,TMB-8从5μmol/L起均抑制ConA介导的淋巴细胞增殖（P〈0.01）,以40μmol/L的效果最为显著,PI值分别为1.01±0.01和1.10±0.01;10μmol/L的TMB-8与25μg/L的环孢菌素A（CsA）具有明显的协同抑制作用（P〈0.01）。细胞周期分析显示,培养48h的TMB-8从10μmol/L起即显著抑制S期（P〈0.01）。结论：TMB-8可明显抑制ConA介导下的T细胞早期活化及增殖,并具T细胞周期的S期阻滞作用。

英文摘要：

AIM： To study the effects of [ 8 - （diethylamino） octyl - 3,4, 5 - trimethoxybenzoate ] （ TMB - 8）, an intracellular Ca^2＋ antagonist, on the activation, proliferation and cell - cycle distribution of the mouse T lymphocytes stimulated by concanvalin A （Con A） in vitro. METHODS： After stimulated with Con A, T cells were treated with different concentrations of TMB -8 alone and its combination with cyclosporine A （CsA）. The expression of CD69, the early marker of CD^3＋ T cell activation, was measured by FACS. The proliferation - related index was determined by carboxyl fluorescin diacetate succinmidyl ester （ CFDA - SE） flow cytometry. The cell - cycle distribution was analyzed by propidium iodide staining. RESULTS： After 6 h culture, the activation rate of CD69^＋ T cell in Con A group was （74.88±1.88） %. 10, 20 and 40 p, mol/L of TMB - 8 inhibited the expression of CD69 （ P 〈 0. 01 ）, especially in 40 μmol/L （52.55% ± 1.54% ）. After 48 h and 72 h culture, the PI of Con A group was 1.24 ± 0.01, 2. 05 ± 0. 07, respectively. TMB - 8 with the concentration up to 5 μmol/L exerted a definite inhibitory effect on the proliferation with a maximal inhi- bition in 40 p, mol/L（P 〈 O. O1 ）. In the combination of 10 p, mol/L of TMB - 8 with 25 p,g/L of CsA, an evident synergistic effect was observed （P 〈 0. 01 ）. Moreover, the cell - cycle distribution analysis showed that after 48 h culture, the concentration of TMB - 8 over 10μmol/L showed an evident suppression in S phase. CONCLUSION ： TMB - 8 significantly inhibites the early steps of the Con A - induced T cell activation and proliferation, as well as the progression of T lymphocytes in S phase.