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棉酚对Jurkat T细胞增殖与凋亡的影响

ISSN号：1674-7666
期刊名称：《中国细胞生物学学报》
时间：0
分类：Q255[生物学—细胞生物学] S858.315.3[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]暨南大学生命科学技术学院组织移植与免疫实验中心,广州510632
相关基金：国家自然科学基金（No.30572199,No.30230350,No.30371651）和生物化学与分子生物学广东省重点学科资助项目

作者：许文彬[1], 徐丽慧[1], 卢宏松[1], 施焕敬[1], 何贤辉[1]

关键词：棉酚, JURKAT, T细胞, 细胞增殖, 凋亡, 流式细胞术, gossypol, Jurkat T cells, proliferation, apoptosis, flow cytometry

中文摘要：

研究棉酚（gossypol）对Jurkat T细胞增殖和凋亡的影响及可能机制。将不同浓度的棉酚作用于Jurkat T细胞，用MTS比色法检测细胞存活率；以膜联蛋白V-PE染色分析细胞凋亡；用Hoechst33342染色观察核形态；用荧光染料Mitocapture结合流式细胞术和激光共焦显微镜检测线粒体跨膜电位变化。结果显示棉酚作用Jurkat T细胞24h、48h、72h，其IC50值分别为77．2μmol／L、57．3μmol／L、23．3μmol／L，对细胞的抑制作用与药物存在时间一剂量依赖关系；终浓度为8、16、32μmol／L的棉酚处理24h后的细胞凋亡率分别为5．30％、15．20％、51．19％，对照组凋亡率为3．43％；高浓度棉酚作用后大量细胞核呈现染色质固缩、核碎裂和致密浓染等凋亡特征；随着浓度增加细胞线粒体跨膜电位明显降低。研究表明棉酚能有效抑制JurkatT细胞增殖和诱导其发生凋亡，并呈现出时间-剂量依赖关系，其诱导凋亡的作用可能依赖于线粒体途径。

英文摘要：

To investigate the effect of gossypol on the proliferation and apoptosis of Jurkat T cells and to elucidate its mechanism. Jurkat T cells were treated with various concentrations of gossypol were assessed. The cell viability was measured by MTS assay. Cell apoptosis was analyzed by labeled with annexin V-PE. Nuclear morphological changes were ascertained by Hoechst33342. Mitochondrial transmembrane potential was detected by Mitocapture combined with flow cytometry （FCM） and laser scanning confocal microscope （LSCM）. Results showed that when cells were treated with gossypol for 24 h, 48 h, 72 h, the IC50 value were about 7.2 μmol/L, 57.3μmol/L, 23.3 μmol/L. The inhibitory effect shows time- and dosedependent. When cells were treated with 8, 16, 32 l.tmol/L of gossypol, the cells apoptosis ratio were about 5.30%, 15.20%, 51.19%, the control group was about 3.43%. With the increase of concentrations, the mitochondrial transmembrane potential was decreased significantly. Many nuclei showed chromatin condensation, nuclear fragmentation and pyknosis after being treated with high concentration of gossypol. The above results indicated that gossypol can effectively inhibit the proliferation of Jurkat T cells and induces apoptosis. The inhibitory effect shows time- and dose- dependent. The mechanism of gossypol induced Jurkat T cells apoptosis probably rely on mitochondria intrinsic pathway.

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