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中文摘要：
目的：研究孕酮（P4）对人外周血来源树突状细胞（DCs）成熟和免疫学功能的影响。方法：在人外周血来源DCs体外培养时加入两种浓度的P4（10^-7mol／L和10^-6mol／L）处理，光镜和电镜下观察DCs的生成情况及形态变化，以流式细胞仪分析各组细胞的免疫表型，用ELISA方法测定其分泌的IL-10和IL-12水平，[^3H]-TdR掺人法检测体外混合淋巴细胞反应中DCs刺激同种反应性T细胞的增殖能力。结果：加入Pd培养后，DCs树突状和膜面状伪足减少，表达低水平MHC-11分子和共刺激分子CD40、CD80和CD86，其分泌的IL-10水平升高，IL-12水平明显下降，DCs刺激同种反应性T细胞的功能显著下降。结论：P4抑制人外周血来源DCs的成熟，对其免疫学功能具有负性调节作用。
英文摘要：
AIM ： To study the effects of progesterone （ P4 ） on the maturation and immunologic function of dendritic cells （DCs） from human peripheral blood. METHODS： Cultured DCs were treated with P4 at doses of 10^-7 mol/L and 10^-6 mol/L. The morphologic changes were observed under the scanning electronic microscope. The immuno-phenotypes of DCs in control and treated groups were analyzed by flow cytometry. IL - 10 and IL - 12 production in culture supernatant was examined by ELISA assay. The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of [ ^3 H] -TdR. RESULTS： Compared with control group, cultured DCs in the presence of P4 displayed less dendritic pseudopod, expressed low levels of MHC - 11, CD40, CD80 and CD86, and exhibited weakly activity in stimulating the proliferation of allogeneic T cells. Increase in IL - 10 production and decrease in IL - 12 production were observed. CONCLUSION： P4 exerts negative effect on the maturation and immunologic function in dendritic cells from human peripheral blood.
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