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中文摘要：
利用超滤膜对珊瑚藻中溴过氧化物酶（EC 1.11.1.18,分子质量740kDa）进行分离纯化,对膜的截留分子质量、操作压力、起始蛋白质浓度、搅拌速率、pH、离子强度等条件进行了优化。超滤分离纯化最优条件为：截留分子质量为100kDa的聚偏氟乙烯（PVDF）膜,操作压力为0.02MPa,搅拌速率为600r/min,起始蛋白质浓度为0.1g/L,pH=6.0。对粗酶液先进行热沉淀纯化,再进行超滤纯化,溴过氧化物酶被纯化了21倍,比活为212U/mg,酶活回收率为96%。
英文摘要：
A novel,simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is developed,which is consist of two steps： crude enzyme extraction was heated at 70℃ for 2h,and then was ultrafiltered with PVDF membrane.The ultrafiltration conditions were optimized,and the optimized conditions are： MWCO=100kDa,0.02MPa pressure,600r/min stirring,0.12mg/ml initial protein concentration and pH=6.0.The crude extraction purified with heat treatment and then ultrafitration results a 21-fold purification,with a 96% overall yield and 212U/mg specific activity.
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