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中文摘要：
利用高效降解纤维素的菌株D1对水葫芦进行预处理，将其内部结构破坏后与活性污泥混合，在不添加任何底物情况下，对比经过预处理与未经预处理水葫芦厌氧发酵产沼气的优化条件，得出最佳优化条件。进一步将水葫芦厌氧发酵产沼气的最佳优化条件进行放大试验，结果表明：在10L厌氧发酵罐内，100g的水葫芦经5mL含有10^7孢子的D1菌株菌悬液预处理48h后，与厌氧活性污泥在30℃厌氧发酵60d左右，其产气效率明显优于未经预处理的水葫芦，达15．52L，平均产气量达155．2mL／g，甲烷含量为63．5％。同时考察了反应器内的pH变化，结果表明pH值相对比较稳定，适合甲烷菌产气。
英文摘要：
Efficient degradation of cellulose Strain D1 was used for water hyacinth pretreatment to destroy its internal structure. After mixed it with the activated sludge with no additional substrate, pretreatment and without pretreatment conditions were compared to obtain optimum conditions for biogas production in water hyacinth anaerobic fermentation. The optimum conditions were amplified. The results showed that water hyacinth of 100 g pretreated by 107 D1 strains of bacteria spore suspension of 5mL for 48 h, then mixed with anaerobic activated sludge of 30℃ , anaerobic fermentation for about 60 d in the anaerobic fermentation reactor of 10 L, whose efficiency of gas production was significantly better than that without pretreatment. The amount of gas production was up to 15.52 L, whose methane content was 63.5%. Changes of the pH in the reactor were also investigated, which showed that pH was relatively stable, suitable for gas production by the methane bacteria.
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