欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
环指蛋白RING1能结合DNA并抑制基因的转录.采用酵母双杂交方法从人骨骼肌文库中筛选出了与A型核纤层蛋白（lamin A）结合的RING1蛋白,回复杂交酵母能在缺陷培养基上生长.RING1与绿色荧光蛋白融合载体转染HEK293细胞,激光共聚焦显微观察发现RING1能与带红色荧光蛋白的lamin A蛋白在细胞核周围共定位.免疫共沉淀结果证明RING1与lamin A能够相互作用.结果证明了一个新的lamin A结合蛋白,为揭示lamin A影响基因表达乃至细胞衰老提供了依据.
英文摘要：
RING1 can bind to DNA and inhibit some gene transcription.RING1 was identified to interact with lamin A by screening a human skeletal muscle library in a yeast two-hybrid interacting screen.The results of one to one reverse hybridization indicated that AH109 transfected with RING1 and lamin A constructs could grow on SD/-Leu/-Trp/-Ade/-His medium.RING1 was inserted into pEGFP-N1 vector and the constructs including pDsRed-LA and pEGFP-RING1 was used to transfect HEK293 cells.Visualized by laser confocal microscopy,RING1 and Lamin A were co-located around nucleus.Interatcion of RING1 and lamin A was comfirmed by immunoprecipitations.These results showed a new lamin A binding protein that can present some evidence for lamin A acting on gene expression and cell senescence.
同期刊论文项目
同项目期刊论文
位置：