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中文摘要：
采用正交试验设计方法，建立了粗毛淫羊藿ISSR分析的优化反应体系：即20μL反应体系中含有10×buffer、2．0mmol·L^-1Mg^2＋、0．10mmol·L^-1 dNTP、1．5UTaq酶、40ng模板DNA和0．6μmol·L^-1引物；PCR反应程序为：95℃5min；94℃30s，52℃45s，72℃2min，共35个循环；72℃10min，4℃保存。
英文摘要：
The optimal ISSR - PCR system in Epimedium acuminatum was established using orthogonal design in the current case. A total volume of 20μL ISSR -PCR system, containing 10 × buffer, 2.0 mmol. L^-1 Mg^2＋ ,0.10 mmol. L^-1 Dntp, 1.5 U Taq polymerase, 40 ng template DNA and 0.6 μmol. L^- 1 primer was proved to better compared with tested cocktails. Further, and the suitable PCR procedure is initiated with one cycle of denaturation at 95℃ for 5 min, followed by 35cycles of denaturing at 94℃ for 30 s, annealing at 52℃ for 45 s, and extending at 72℃ for 2 min, then finally extended at 72℃ for 10 min. The amplified mixture is deposited at 4℃.
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