目的:检测凋亡抑制因子Survivin mRNA在肝癌组织中的表达.探讨Survivin mRNA在肝癌诊断及治疗方面的应用。方法:采用逆转录多聚酶联反应(RT—PCR)技术检测46例肝癌组织、30例肝硬化组织及10例正常肝脏组织中Survivin mRNA的表达。结果:46例肝癌组织中Survivin mRNA阳性表达率为100%,而在30例肝硬化活检病理标本和10例正常肝脏组织中未表达。结论:Survivin mRNA有望成为新的肿瘤标志物,应用于临床以协助肝癌诊断和靶向性基因治疗。
Objective:To study the expression and significance of Survivin mRNA in tissues of human hepatocellular carcinoma (HCC). Method: Reverse transcriptase polymerase chain reaction (RT-PCR)was used to detect the expression of Survivin mRNA in 46 HCC speciments,30 liver cirrhosis speciments and 10 healthy liver speciments. Result:Survivin mRNA was detectable in 46 HCC speciments,but was not detectable in 30 liver cirrhosis speciments and 10 healthy liver speciments.Conclusion:Survivin mRNA could become a new cancer biomarker for diagnosis and targeted gene therapy of hepatocellular carcinoma.